論文 - 詳細
| RRC ID | 61217 |
|---|---|
| 著者 | Ohno T, Saijo-Kurita K, Miyamoto-Eimori N, Kurose T, Aoki Y, Yosimura S. |
| タイトル | A simple method for in situ freezing of anchorage-dependent cells including rat liver parenchymal cells. |
| ジャーナル | Cytotechnology |
| Abstract |
We developed a simple method for freezing anchorage-dependent cells, including primary cultured rat liver parenchymal cells, without detaching the cells from the culture dish. The method consists of preculture of the cells to confluence, changing the growth medium to a conventional freezing medium, packaging in a container, and storage at -80 degrees C. After thawing and changing the freezing medium to regular growth medium, cell growth was nearly identical to that of cells freshly seeded into a new dish. |
| 巻・号 | 5(3) |
| ページ | 273-7 |
| 公開日 | 1991-3-1 |
| DOI | 10.1007/BF00556297 |
| PMID | 1367380 |
| MeSH | Animals Cell Adhesion Cell Division Cell Line Cells, Cultured Cryopreservation / instrumentation Cryopreservation / methods* Freezing* Liver / cytology* Mice Mice, Inbred BALB C Rats Time Factors Vero Cells |
| IF | 1.777 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 5 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | Vero(RCB0001) |