RRC ID 61221
Author Uemura T, Takamatsu K, Ikeda M, Okada M, Kazuki K, Ikada Y, Nakamura H.
Title A tissue-engineered bioabsorbable nerve conduit created by three-dimensional culture of induced pluripotent stem cell-derived neurospheres.
Journal Biomed Mater Eng
Abstract We previously reported a bioabsorbable nerve conduit coated with Schwann cells for the treatment of peripheral nerve defects. Since there have been dramatic developments in induced pluripotent stem (iPS) cells in recent years, the purpose of the present study was to create a tissue-engineered nerve conduit coated with iPS cell-derived neurospheres. Such a conduit was constructed by three-dimensional (3D)-culture of these cells using a bioabsorbable polymer conduit as a scaffold. The nerve conduit was composed of a mesh of poly L-lactide, and a porous sponge of 50% poly L-lactide and 50% poly ε-caprolactone. The primary and secondary neurospheres (PNS and SNS, respectively) induced from iPS cells were suspended in individual conduits. The conduits were incubated for 7 or 14 days in vitro and then evaluated using immunohistochemistry. All of the 7- and 14-day differentiated PNS and SNS were observed to have adhered to the inner surface of the conduits and to have migrated into the inner porous sponge. The engrafted cells were positive for anti-Tuj1, -S-100 and -GFAP antibodies, indicating that their pluripotent ability to form neural or glial cells was maintained. These findings indicate the feasibility of creating nerve conduits coated with a 3D-culture of iPS cell-derived neurospheres for the treatment of peripheral nerve defects.
Volume 21(5-6)
Pages 333-9
Published 2011-1-1
DOI 10.3233/BME-2012-0680
PII Y008T5658H438M8H
PMID 22561252
MeSH Absorbable Implants* Cell Culture Techniques / instrumentation Cell Culture Techniques / methods Cell Differentiation / physiology Cells, Cultured Guided Tissue Regeneration / instrumentation* Humans Induced Pluripotent Stem Cells / cytology Induced Pluripotent Stem Cells / physiology* Nerve Regeneration* / drug effects Nerve Regeneration* / physiology Neurons / cytology* Neurons / physiology Polymers / chemistry Polymers / pharmacology Porosity Spheroids, Cellular / cytology Spheroids, Cellular / physiology Tissue Engineering* / methods Tissue Scaffolds / chemistry
IF 1.243
Human and Animal Cells iPS-MEF-Ng-178B-5(APS0002)