| RRC ID |
61221
|
| 著者 |
Uemura T, Takamatsu K, Ikeda M, Okada M, Kazuki K, Ikada Y, Nakamura H.
|
| タイトル |
A tissue-engineered bioabsorbable nerve conduit created by three-dimensional culture of induced pluripotent stem cell-derived neurospheres.
|
| ジャーナル |
Biomed Mater Eng
|
| Abstract |
We previously reported a bioabsorbable nerve conduit coated with Schwann cells for the treatment of peripheral nerve defects. Since there have been dramatic developments in induced pluripotent stem (iPS) cells in recent years, the purpose of the present study was to create a tissue-engineered nerve conduit coated with iPS cell-derived neurospheres. Such a conduit was constructed by three-dimensional (3D)-culture of these cells using a bioabsorbable polymer conduit as a scaffold. The nerve conduit was composed of a mesh of poly L-lactide, and a porous sponge of 50% poly L-lactide and 50% poly ε-caprolactone. The primary and secondary neurospheres (PNS and SNS, respectively) induced from iPS cells were suspended in individual conduits. The conduits were incubated for 7 or 14 days in vitro and then evaluated using immunohistochemistry. All of the 7- and 14-day differentiated PNS and SNS were observed to have adhered to the inner surface of the conduits and to have migrated into the inner porous sponge. The engrafted cells were positive for anti-Tuj1, -S-100 and -GFAP antibodies, indicating that their pluripotent ability to form neural or glial cells was maintained. These findings indicate the feasibility of creating nerve conduits coated with a 3D-culture of iPS cell-derived neurospheres for the treatment of peripheral nerve defects.
|
| 巻・号 |
21(5-6)
|
| ページ |
333-9
|
| 公開日 |
2011-1-1
|
| DOI |
10.3233/BME-2012-0680
|
| PII |
Y008T5658H438M8H
|
| PMID |
22561252
|
| MeSH |
Absorbable Implants*
Cell Culture Techniques / instrumentation
Cell Culture Techniques / methods
Cell Differentiation / physiology
Cells, Cultured
Guided Tissue Regeneration / instrumentation*
Humans
Induced Pluripotent Stem Cells / cytology
Induced Pluripotent Stem Cells / physiology*
Nerve Regeneration* / drug effects
Nerve Regeneration* / physiology
Neurons / cytology*
Neurons / physiology
Polymers / chemistry
Polymers / pharmacology
Porosity
Spheroids, Cellular / cytology
Spheroids, Cellular / physiology
Tissue Engineering* / methods
Tissue Scaffolds / chemistry
|
| IF |
1.243
|
| リソース情報 |
| ヒト・動物細胞 |
iPS-MEF-Ng-178B-5(APS0002) |
