Reference - Detail
| RRC ID | 61224 |
|---|---|
| Author | Jawhari AU, Noda M, Farthing MJ, Pignatelli M. |
| Title | Abnormal expression and function of the E-cadherin-catenin complex in gastric carcinoma cell lines. |
| Journal | Br J Cancer |
| Abstract |
Dysfunction of the cadherin-catenin complex, a key component of adherens junctions, is thought to confer invasive potential to cells. The aim of this study is to examine the expression and function of the E-cadherin/catenin complex in gastric carcinoma cell lines. Expression of E-cadherin, alpha, beta and gamma-catenin and p120ctn, and of the adenomatous polyposis coli protein (APC), together with function of the cadherin-catenin complex was examined in a panel of gastric carcinoma cell lines, using immunocytochemistry, Western blotting and a cell-cell aggregation assay. Protein interactions were examined by sequential immunoprecipitation and immunoblotting with antibodies to E-cadherin, alpha, beta and gamma-catenin, p120ctn and APC. Abnormalities of E-cadherin, alpha- and beta-catenin expression, were associated with disturbance of E-cadherin-catenin complex composition, loss of membranous localization and loss of calcium-dependent aggregation in six gastric carcinoma cell lines. APC protein expression and interaction with beta-catenin was preserved in five cell lines. We demonstrate frequent abnormalities of expression and function of E-cadherin and catenins, and associated disturbance of E-cadherin-mediated intercellular adhesion in gastric carcinoma cell lines. These findings support the tumour suppressor role of the E-cadherin and its contribution to the development and progression of the neoplastic phenotype in gastric carcinoma. |
| Volume | 80(3-4) |
| Pages | 322-30 |
| Published | 1999-5-1 |
| DOI | 10.1038/sj.bjc.6690358 |
| PII | 6690358 |
| PMID | 10408833 |
| PMC | PMC2362351 |
| MeSH | Animals Cadherins / biosynthesis* Cadherins / metabolism Cadherins / physiology Carcinoma / metabolism* Carcinoma / pathology Cell Adhesion / physiology Cell Aggregation / physiology Cell Communication / physiology Cytoskeletal Proteins / biosynthesis* Cytoskeletal Proteins / metabolism Cytoskeletal Proteins / physiology Humans Macromolecular Substances Mice Mice, Inbred BALB C Mice, Nude Stomach Neoplasms / metabolism* Stomach Neoplasms / pathology Trans-Activators* Tumor Cells, Cultured alpha Catenin beta Catenin |
| IF | 5.791 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Peer-review(Publons) |
| Total number of mentions | 4 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | MKN7(RCB0999) MKN74(RCB1002) |