RRC ID 6124
Author Sakudoh T, Iizuka T, Narukawa J, Sezutsu H, Kobayashi I, Kuwazaki S, Banno Y, Kitamura A, Sugiyama H, Takada N, Fujimoto H, Kadono-Okuda K, Mita K, Tamura T, Yamamoto K, Tsuchida K.
Title A CD36-related transmembrane protein is coordinated with an intracellular lipid-binding protein in selective carotenoid transport for cocoon coloration.
Journal J Biol Chem
Abstract The transport pathway of specific dietary carotenoids from the midgut lumen to the silk gland in the silkworm, Bombyx mori, is a model system for selective carotenoid transport because several genetic mutants with defects in parts of this pathway have been identified that manifest altered cocoon pigmentation. In the wild-type silkworm, which has both genes, Yellow blood (Y) and Yellow cocoon (C), lutein is transferred selectively from the hemolymph lipoprotein to the silk gland cells where it is accumulated into the cocoon. The Y gene encodes an intracellular carotenoid-binding protein (CBP) containing a lipid-binding domain known as the steroidogenic acute regulatory protein-related lipid transfer domain. Positional cloning and transgenic rescue experiments revealed that the C gene encodes Cameo2, a transmembrane protein gene belonging to the CD36 family genes, some of which, such as the mammalian SR-BI and the fruit fly ninaD, are reported as lipoprotein receptors or implicated in carotenoid transport for visual system. In C mutant larvae, Cameo2 expression was strongly repressed in the silk gland in a specific manner, resulting in colorless silk glands and white cocoons. The developmental profile of Cameo2 expression, CBP expression, and lutein pigmentation in the silk gland of the yellow cocoon strain were correlated. We hypothesize that selective delivery of lutein to specific tissue requires the combination of two components: 1) CBP as a carotenoid transporter in cytosol and 2) Cameo2 as a transmembrane receptor on the surface of the cells.
Volume 285(10)
Pages 7739-51
Published 2010-3-5
DOI 10.1074/jbc.M109.074435
PII M109.074435
PMID 20053988
PMC PMC2844218
MeSH Amino Acid Sequence Animals Bombyx* / anatomy & histology Bombyx* / genetics Bombyx* / metabolism CD36 Antigens / genetics CD36 Antigens / metabolism Carotenoids / metabolism* Carrier Proteins / genetics Carrier Proteins / metabolism Chromosome Mapping Fatty Acid-Binding Proteins / genetics Fatty Acid-Binding Proteins / metabolism* Female Gene Expression Regulation, Developmental Genetic Linkage Insect Proteins / genetics Insect Proteins / metabolism* Lutein / chemistry Lutein / metabolism Male Membrane Proteins / genetics Membrane Proteins / metabolism* Molecular Sequence Data Pigmentation / physiology* Sequence Homology, Amino Acid Silk / chemistry* Silk / metabolism Transgenes
IF 4.106
Times Cited 51
Silkworms c04 c05 c10 c11 c43 e09