論文 - 詳細
| RRC ID | 61252 |
|---|---|
| 著者 | Saito Y, Kasamatsu A, Yamamoto A, Shimizu T, Yokoe H, Sakamoto Y, Ogawara K, Shiiba M, Tanzawa H, Uzawa K. |
| タイトル | ALY as a potential contributor to metastasis in human oral squamous cell carcinoma. |
| ジャーナル | J Cancer Res Clin Oncol |
| Abstract |
PURPOSE:ALY, an essential mRNA export factor, is dysregulated in a wide variety of human malignancies. However, little is known about the relevance of ALY to oral squamous cell carcinoma (OSCC). The purpose of this study was to investigate ALY expression and its functional mechanisms in OSCCs. METHODS:ALY mRNA and protein expression in seven OSCC-derived cell lines (Sa3, HO-1-u-1, KON, Ca9-22, HSC-2, HSC-3, and HSC-4) and primary OSCCs were analyzed by quantitative reverse transcriptase-polymerase chain reaction, immunoblotting, and immunohistochemistry. We evaluated cellular invasiveness, migration, and the expression levels of metastasis modulators, ribosomal RNA processing 1 homolog B (RRP1B) and CD82, in ALY knockdown cells. RESULTS:ALY was frequently up-regulated in OSCC-derived cell lines and primary OSCCs compared with normal counterparts at both the mRNA and protein expression levels. ALY-positive expression was correlated significantly (P < 0.05) with a higher risk of regional lymph node metastasis. Furthermore, ALY knockdown cells caused a significant (P < 0.05) decrease in cellular invasiveness and migration with up-regulation of RRP1B and CD82 compared with the control cells. CONCLUSION:Our results showed that ALY is linked to regional lymph node metastasis by regulating cellular invasiveness and migration. Therefore, ALY might be a potential biomarker for early detection of lymph node metastasis in OSCCs. |
| 巻・号 | 139(4) |
| ページ | 585-94 |
| 公開日 | 2013-4-1 |
| DOI | 10.1007/s00432-012-1361-5 |
| PMID | 23242234 |
| MeSH | Apoptosis Biomarkers, Tumor / genetics* Biomarkers, Tumor / metabolism Blotting, Western Carcinoma, Squamous Cell / diagnosis* Carcinoma, Squamous Cell / genetics Carcinoma, Squamous Cell / metabolism Case-Control Studies Cell Adhesion Cell Movement Cell Proliferation Female Humans Immunoenzyme Techniques Lymphatic Metastasis Male Middle Aged Mouth Mucosa / metabolism Mouth Mucosa / pathology Mouth Neoplasms / diagnosis* Mouth Neoplasms / genetics Mouth Neoplasms / metabolism Neoplasm Staging Nuclear Proteins / antagonists & inhibitors Nuclear Proteins / genetics Nuclear Proteins / metabolism* Prognosis RNA, Messenger / genetics RNA, Small Interfering / genetics RNA-Binding Proteins / antagonists & inhibitors RNA-Binding Proteins / genetics RNA-Binding Proteins / metabolism* Real-Time Polymerase Chain Reaction Reverse Transcriptase Polymerase Chain Reaction Transcription Factors / antagonists & inhibitors Transcription Factors / genetics Transcription Factors / metabolism* Tumor Cells, Cultured |
| IF | 3.656 |
| リソース情報 | |
| ヒト・動物細胞 | Sa3(RCB0980) HSC-2(RCB1945) HSC-4(RCB1902) HO-1-u-1(RCB2102) Ca9-22(RCB1976) |