論文 - 詳細
| RRC ID | 61253 |
|---|---|
| 著者 | Mitani K, Haruyama N, Hatakeyama J, Igarashi K. |
| タイトル | Amelogenin splice isoforms stimulate chondrogenic differentiation of ATDC5 cells. |
| ジャーナル | Oral Dis |
| Abstract |
OBJECTIVE:Amelogenins are the most abundant matrix proteins in enamel. Among the amelogenin isoforms, full-length amelogenin (M180) and leucine-rich amelogenin peptide (LRAP) are expressed in various tissues and are implicated as signalling molecules in mesenchymal cells. Here, we examined the effects of M180 and LRAP on a chondrogenic cell line, ATDC5, to investigate the role of amelogenins in chondrogenesis. MATERIALS AND METHODS:Recombinant mouse M180- or LRAP-protein-containing medium or control medium was mixed with a chondrogenesis-stimulating medium, and changes in the phenotype, gene expression levels and cell proliferation of cultured ATDC5 cells were analysed. RESULTS:The addition of amelogenins increased alkaline phosphatase activity and glycosaminoglycan secretion at 14 and 21 days of culture, respectively, as compared with the control. Quantitative PCR (Q-PCR) analysis revealed that LRAP increased the gene expression levels of Runx2, Col2a1 and Aggrecan at 7 days of differentiation. Moreover, both M180 and LRAP significantly increased the gene expression levels of ALP, Aggrecan, Col10a1 and osteopontin at 28 days of culture. Bromodeoxyuridine assay and Q-PCR analysis for Wnt signalling indicated that both M180 and LRAP reduced proliferation, but induced the cell differentiation possibly through altered non-canonical Wnt signalling. CONCLUSION:M180 and LRAP accelerate chondrogenic differentiation and maturation of ATDC5 cells. |
| 巻・号 | 19(2) |
| ページ | 169-79 |
| 公開日 | 2013-3-1 |
| DOI | 10.1111/j.1601-0825.2012.01967.x |
| PMID | 22863294 |
| MeSH | Amelogenin / physiology* Animals Cell Differentiation Cell Line Cells, Cultured Chondrogenesis / physiology* Dental Enamel Proteins / physiology* Mice Protein Isoforms |
| IF | 2.613 |
| リソース情報 | |
| ヒト・動物細胞 | ATDC5(RCB0565) MC3T3-E1(RCB1126) |