| RRC ID |
61267
|
| 著者 |
Inada M, Katagiri T, Akiyama S, Namika M, Komaki M, Yamaguchi A, Kamoi K, Rosen V, Suda T.
|
| タイトル |
Bone morphogenetic protein-12 and -13 inhibit terminal differentiation of myoblasts, but do not induce their differentiation into osteoblasts.
|
| ジャーナル |
Biochem Biophys Res Commun
|
| Abstract |
Effects of bone morphogenetic protein (BMP)-12 and BMP-13, new members of the BMP family which belong to the transforming growth factor (TGF)-beta superfamily, on terminal differentiation of myoblasts were examined in C2C12 and L-6 myoblasts. When the myoblasts were cultured with BMP-12 or BMP-13, the expression of the myosin heavy chain and the formation of multinucleated myotubes mRNA in L-6 cells. The inhibitory effects of BMP-12 and BMP-13 on myogenic differentiation were similar to the effects of BMP-2, though their potencies were lower than BMP-2. Unlike BMP-2, neither BMP-12 nor BMP-13 induced alkaline phosphatase activity in C2C12 myoblasts. The differences in the biological activities of these new BMPs suggest that the intracellular signalling pathway used by BMP-12 and BMP-13 differs from that of BMP-2.
|
| 巻・号 |
222(2)
|
| ページ |
317-22
|
| 公開日 |
1996-5-15
|
| DOI |
10.1006/bbrc.1996.0742
|
| PII |
S0006291X96907421
|
| PMID |
8670203
|
| MeSH |
Alkaline Phosphatase / biosynthesis
Animals
Bone Morphogenetic Proteins
Cell Differentiation / drug effects*
Dose-Response Relationship, Drug
Enzyme Induction
Humans
Kinetics
Mice
Muscle, Skeletal / cytology*
Muscle, Skeletal / drug effects
Myosin Heavy Chains / biosynthesis
Osteoblasts / cytology*
Osteoblasts / drug effects
Proteins / pharmacology*
RNA, Messenger / biosynthesis
Recombinant Proteins / pharmacology
Transforming Growth Factor beta / pharmacology
|
| IF |
2.985
|
| リソース情報 |
| ヒト・動物細胞 |
ROS-17/2.8-5(RCB0462) |
