RRC ID 61269
Author Sasaki K, Yoneda A, Ninomiya A, Kawahara M, Watanabe T.
Title Both antiviral activity and intracellular localization of chicken Mx protein depend on a polymorphism at amino acid position 631.
Journal Biochem Biophys Res Commun
Abstract The Mx protein is known to inhibit the multiplication of several RNA viruses. In chickens, a polymorphism at amino acid position 631 (631 aa) of Mx protein has been suggested to be involved in the antiviral ability against vesicular stomatitis virus (VSV) and influenza virus, indicating that a Ser-to-Asn substitution at 631 aa is the source of this antiviral ability. However, how the substitution at 631 aa contributes to the antiviral activity remains to be clarified. In this study, we investigated differences in antiviral activity against VSV and intracellular localization between Ser and Asn types at 631 aa of the chicken Mx protein. The results showed that chicken Mx protein with an Asn at 631 aa inhibited VSV multiplication and Mx distribution in a granular-like pattern in the cytoplasm. However, Mx carrying the Ser type did not inhibit viral growth and homogenous spread throughout the cytoplasm. Furthermore, we found that replacing Ser with Asn at 631 aa provided Mx with antiviral activity against VSV, with Mx showing granular-like distribution in the cytoplasm. These results demonstrated that a single amino acid polymorphism at 631 aa of the chicken Mx protein altered both the antiviral activity and intracellular localization.
Volume 430(1)
Pages 161-6
Published 2013-1-4
DOI 10.1016/j.bbrc.2012.11.053
PII S0006-291X(12)02228-0
PMID 23201406
MeSH 3T3 Cells Animals Chickens / immunology* Cytoplasm / metabolism* GTP-Binding Proteins / genetics GTP-Binding Proteins / metabolism* Mice Myxovirus Resistance Proteins Orthomyxoviridae / immunology* Orthomyxoviridae Infections / immunology Polymorphism, Genetic Rhabdoviridae Infections / immunology Vesicular stomatitis Indiana virus / immunology*
IF 2.985
Human and Animal Cells RGB3T3-1(RCB0163)