| RRC ID |
613
|
| 著者 |
Dezar CA, Gago GM, Gonzalez DH, Chan RL.
|
| タイトル |
Hahb-4, a sunflower homeobox-leucine zipper gene, is a developmental regulator and confers drought tolerance to Arabidopsis thaliana plants.
|
| ジャーナル |
Transgenic Res
|
| Abstract |
Homeodomain-leucine zipper proteins constitute a family of transcription factors found only in plants. Hahb-4 is a member of Helianthus annuus (sunflower) subfamily I. It is regulated at the transcriptional level by water availability and abscisic acid. In order to establish if this gene plays a functional role in drought responses, transgenic Arabidopsis thaliana plants that overexpress Hahb-4 under the control of the 35S Cauliflower Mosaic Virus promoter were obtained. Transformed plants show a specific phenotype: they develop shorter stems and internodes, rounder leaves and more compact inflorescences than their non-transformed counterparts. Shorter stems and internodes are due to a lower rate in cell elongation rather than to a stop in cell division. Transgenic plants were more tolerant to water stress conditions, showing improved development, a healthier appearance and higher survival rates than wild-type plants. Indeed, either under normal or drought conditions, they produce approximately the same seed weight per plant as wild-type plants under normal growth conditions. Plants transformed with a construct that bears the Hahb-4 promoter fused to gusA show reporter gene expression in defined cell-types and developmental stages and are induced by drought and abscisic acid. Since Hahb-4 is a transcription factor, we propose that it may participate in the regulation of the expression of genes involved in developmental responses of plants to desiccation.
|
| 巻・号 |
14(4)
|
| ページ |
429-40
|
| 公開日 |
2005-8-1
|
| DOI |
10.1007/s11248-005-5076-0
|
| PMID |
16201409
|
| MeSH |
Arabidopsis / genetics*
Arabidopsis / physiology
Arabidopsis Proteins / genetics
Arabidopsis Proteins / metabolism
Crops, Agricultural / genetics
Crops, Agricultural / physiology
DNA, Plant / isolation & purification
Disasters
Gene Expression Regulation, Plant
Genes, Homeobox / physiology*
Genes, Plant
Helianthus / genetics
Homeodomain Proteins
Leucine Zippers / genetics*
Phenotype
Plant Growth Regulators / genetics*
Plants, Genetically Modified / genetics
Promoter Regions, Genetic
RNA, Plant / isolation & purification
RNA, Plant / metabolism
Transcription Factors
|
| IF |
1.856
|
| 引用数 |
90
|
|
WOS 分野
|
BIOCHEMICAL RESEARCH METHODS
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| シロイヌナズナ / 植物培養細胞・遺伝子 |
pda01352
pda07022 |
