Reference - Detail
| RRC ID | 61300 |
|---|---|
| Author | Hida K, Shindoh M, Yasuda M, Hanzawa M, Funaoka K, Kohgo T, Amemiya A, Totsuka Y, Yoshida K, Fujinaga K. |
| Title | Antisense E1AF transfection restrains oral cancer invasion by reducing matrix metalloproteinase activities. |
| Journal | Am J Pathol |
| Abstract |
E1AF is a newly identified human ets-family transcription factor. We have reported that E1AF can up-regulate transcription of matrix metalloproteinase (MMP) genes and confers invasive phenotype on human cancer cells. HSC3 is an oral squamous-cell-carcinoma-derived cell line, and it manifests high levels of E1AF and MMP-1 and -9 gene expression that are associated with invasive potential. We reconstructed an E1AF antisense expression vector, transfected HSC3 cells with the vector, and obtained HSC3AS cells that express E1AF antisense RNA. HSC3AS showed decreasing mRNA and protein levels of MMP-1, -3, and -9. Moreover, HSC3AS showed lower invasive potential in vitro three-dimensional raft culture and in vivo implantation into nude mice. These results imply that transfection of antisense E1AF inhibits tumor invasion by down-regulating MMP genes. |
| Volume | 150(6) |
| Pages | 2125-32 |
| Published | 1997-6-1 |
| PMID | 9176403 |
| PMC | PMC1858336 |
| MeSH | Adenovirus E1A Proteins / genetics* Animals Blotting, Northern Carcinoma, Squamous Cell / enzymology* Carcinoma, Squamous Cell / genetics* Carcinoma, Squamous Cell / pathology Cell Culture Techniques / methods Collagenases / metabolism Humans Immunohistochemistry Matrix Metalloproteinase 1 Matrix Metalloproteinase 3 / metabolism Matrix Metalloproteinase 9 Metalloendopeptidases / metabolism* Mice Mice, Nude Mouth Neoplasms / enzymology* Mouth Neoplasms / genetics* Mouth Neoplasms / pathology Neoplasm Invasiveness / genetics* Neoplasm Transplantation Proto-Oncogene Proteins / genetics* Proto-Oncogene Proteins c-ets RNA, Antisense / genetics RNA, Messenger / metabolism Transcription Factors / genetics* Transfection / genetics Tumor Cells, Cultured |
| IF | 3.491 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 4 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | MRC-5(RCB0211) MRC-5(RCb0218) |