Reference - Detail
| RRC ID | 61322 |
|---|---|
| Author | Kamao H, Mandai M, Okamoto S, Sakai N, Suga A, Sugita S, Kiryu J, Takahashi M. |
| Title | Characterization of human induced pluripotent stem cell-derived retinal pigment epithelium cell sheets aiming for clinical application. |
| Journal | Stem Cell Reports |
| Abstract |
Age-related macular degeneration (AMD) causes severe visual impairment due in part to age-dependent impairment of retinal pigment epithelium (RPE). It has been suggested that autologous human induced pluripotent stem cells (hiPSCs) may represent a useful cell source for the generation of graft RPE. We generated hiPSC-derived RPE (hiPSC-RPE) cell sheets optimized to meet clinical use requirements, including quality, quantity, consistency, and safety. These cell sheets are generated as a monolayer of cells without any artificial scaffolds, express typical RPE markers, form tight junctions that exhibit polarized secretion of growth factors, and show phagocytotic ability and gene-expression patterns similar to those of native RPE. Additionally, upon transplantation, autologous nonhuman primate iPSC-RPE cell sheets showed no immune rejection or tumor formation. These results suggest that autologous hiPSC-RPE cell sheets may serve as a useful form of graft for use in tissue replacement therapy for AMD. |
| Volume | 2(2) |
| Pages | 205-18 |
| Published | 2014-2-11 |
| DOI | 10.1016/j.stemcr.2013.12.007 |
| PII | S2213-6711(13)00175-6 |
| PMID | 24527394 |
| PMC | PMC3923225 |
| MeSH | Animals Biomarkers / metabolism Cell Culture Techniques* Cell Differentiation Cluster Analysis Epithelial Cells / cytology* Epithelial Cells / immunology Epithelial Cells / metabolism Epithelial Cells / transplantation Gene Expression Profiling Humans Induced Pluripotent Stem Cells / cytology* Macaca fascicularis Rats Retinal Pigment Epithelium / cytology* |
| IF | 6.032 |
| Altmetric score |
オルトメトリクス指標項目
|
| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 114 |
| Altmetric score changes over past 6months | 3.0 |
| Resource | |
| Human and Animal Cells | 253G1(HPS0002) |