RRC ID 61325
Author Nakashima T, Tamura T, Kurachi M, Yamaguchi K, Oda T.
Title Apoptosis-mediated cytotoxicity of prodigiosin-like red pigment produced by gamma-Proteobacterium and its multiple bioactivities.
Journal Biol Pharm Bull
Abstract Recently we discovered a bacterial strain (MS-02-063) that produces large amounts of red pigment (PG-L-1). Among the cell lines tested, U937 cells showed the highest susceptibility to PG-L-1 toxicity. PG-L-1 induced typical apoptotic nuclear morphological changes, and single cell gel electrophoresis revealed that PG-L-1 caused DNA fragmentation in U937 cells. In PG-L-1 treated U937 cells, the acidic compartment such as lysosomes disappeared, suggesting that PG-L-1-induced disorder of intracellular pH compartmentalization might trigger apoptotic signal. Since p38 MAP kinase inhibitor specifically prevented the PG-L-1 mediated cell death, p38 MAP kinase may be involved in the cytotoxic mechanism. In fact, immunoblot analysis of p38 MAP kinase revealed that phosphorylation of p38 MAP kinase occurred in PG-L-1-treated U937 cells. In addition to the activity to induce apoptotic cell death as reported in several PG family members, our chemiluminescence analysis suggested that PG-L-1 inhibited superoxide generation by 12-O-tetradecanoylphorbol-13-acetate (TPA)-stimulated U937 cells in a dose-dependent manner. Since PG-L-1 had no effect on the chemiluminescence response caused by xanthine oxidase/hypoxanthine system, PG-L-1 acts on the enzyme system responsible for O(2)(-) generation rather than direct scavenging toward O(2)(-). Our results suggest that PG-L-1 causes multiple biochemical effects on the target cells such as increase in pH in acidic intracellular compartment, activation of p38 MAP kinase, inhibition of O(2)(-) generation, and eventually induces apoptotic cell death.
Volume 28(12)
Pages 2289-95
Published 2005-12-1
DOI 10.1248/bpb.28.2289
PII JST.JSTAGE/bpb/28.2289
PMID 16327167
MeSH Acridine Orange Anti-Bacterial Agents / pharmacology* Anti-Bacterial Agents / toxicity Apoptosis* Cell Compartmentation / drug effects Cell Survival / drug effects DNA Fragmentation / drug effects Dose-Response Relationship, Drug Gammaproteobacteria / metabolism* Humans Hydrogen-Ion Concentration Immunoblotting Luminescent Measurements Prodigiosin / analogs & derivatives* Prodigiosin / metabolism Prodigiosin / toxicity* Serratia marcescens / metabolism Superoxides / antagonists & inhibitors Superoxides / metabolism Tetradecanoylphorbol Acetate / pharmacology U937 Cells / drug effects U937 Cells / metabolism U937 Cells / ultrastructure p38 Mitogen-Activated Protein Kinases / drug effects p38 Mitogen-Activated Protein Kinases / metabolism
IF 1.863
Human and Animal Cells MDCK(RCB0995) HeLa(RCB0007) Vero(RCB0001)