RRC ID 61328
著者 Rahimi M, Youn HY, McCanna DJ, Sivak JG, Mikkelsen SR.
タイトル Application of cyclic biamperometry to viability and cytotoxicity assessment in human corneal epithelial cells.
ジャーナル Anal Bioanal Chem
Abstract The application of cyclic biamperometry to viability and cytotoxicity assessments of human corneal epithelial cells has been investigated. Electrochemical measurements have been compared in PBS containing 5.0 mM glucose and minimal essential growth medium. Three different lipophilic mediators including dichlorophenol indophenol, 2-methyl-1,4-naphthoquinone (also called menadione or vitamin K3) and N,N,N',N'-tetramethyl-p-phenylenediamine have been evaluated for shuttling electrons across the cell membrane to the external medium. Transfer of these electrons to ferricyanide in the extra cellular medium results in the accumulation of ferrocyanide. The amount of ferrocyanide is then determined using cyclic biamperometry and is related to the extent of cell metabolic activity and therefore cell viability. To illustrate cytotoxicity assessment of chemicals, hydrogen peroxide, benzalkonium chloride and sodium dodecyl sulfate have been chosen as sample toxins, the cytotoxicities of which have been evaluated and compared to values reported in the literature. Similar values have been reported using colorimetric assays; however, the simplicity of this electrochemical assay can, in principle, open the way to miniaturization onto lab-on-chip devices and its incorporation into tiered-testing approaches for cytotoxicity assessment.
巻・号 405(14)
ページ 4975-9
公開日 2013-5-1
DOI 10.1007/s00216-013-6843-z
PMID 23443523
MeSH Biological Assay / instrumentation* Cell Survival / drug effects Cell Survival / physiology Cells, Cultured Conductometry / instrumentation* Epithelial Cells / cytology Epithelial Cells / drug effects* Epithelial Cells / physiology* Epithelium, Corneal / cytology Epithelium, Corneal / drug effects* Epithelium, Corneal / physiology* Equipment Design Equipment Failure Analysis Reproducibility of Results Sensitivity and Specificity Toxicity Tests / instrumentation*
IF 3.637
リソース情報
ヒト・動物細胞 HCE-T(RCB2280)