RRC ID 6137
Author Kuroda Y, Kitada M, Wakao S, Nishikawa K, Tanimura Y, Makinoshima H, Goda M, Akashi H, Inutsuka A, Niwa A, Shigemoto T, Nabeshima Y, Nakahata T, Nabeshima Y, Fujiyoshi Y, Dezawa M.
Title Unique multipotent cells in adult human mesenchymal cell populations.
Journal Proc. Natl. Acad. Sci. U.S.A.
Abstract We found adult human stem cells that can generate, from a single cell, cells with the characteristics of the three germ layers. The cells are stress-tolerant and can be isolated from cultured skin fibroblasts or bone marrow stromal cells, or directly from bone marrow aspirates. These cells can self-renew; form characteristic cell clusters in suspension culture that express a set of genes associated with pluripotency; and can differentiate into endodermal, ectodermal, and mesodermal cells both in vitro and in vivo. When transplanted into immunodeficient mice by local or i.v. injection, the cells integrated into damaged skin, muscle, or liver and differentiated into cytokeratin 14-, dystrophin-, or albumin-positive cells in the respective tissues. Furthermore, they can be efficiently isolated as SSEA-3(+) cells. Unlike authentic ES cells, their proliferation activity is not very high and they do not form teratomas in immunodeficient mouse testes. Thus, nontumorigenic stem cells with the ability to generate the multiple cell types of the three germ layers can be obtained through easily accessible adult human mesenchymal cells without introducing exogenous genes. These unique cells will be beneficial for cell-based therapy and biomedical research.
Volume 107(19)
Pages 8639-43
Published 2010-5-11
DOI 10.1073/pnas.0911647107
PII 0911647107
PMID 20421459
PMC PMC2889306
MeSH Adult Adult Stem Cells / cytology* Animals Bone Marrow Cells / cytology Cell Aggregation Cell Differentiation Cell Proliferation Humans Mesenchymal Stem Cell Transplantation Mesenchymal Stromal Cells / cytology* Mice Multipotent Stem Cells / cytology*
IF 9.58
Times Cited 172
Human embryonic stem cells KhES-1