論文 - 詳細
| RRC ID | 614 |
|---|---|
| 著者 | Pourcel L, Routaboul JM, Kerhoas L, Caboche M, Lepiniec L, Debeaujon I. |
| タイトル | TRANSPARENT TESTA10 encodes a laccase-like enzyme involved in oxidative polymerization of flavonoids in Arabidopsis seed coat. |
| ジャーナル | Plant Cell |
| Abstract |
The Arabidopsis thaliana transparent testa10 (tt10) mutant exhibits a delay in developmentally determined browning of the seed coat, also called the testa. Seed coat browning is caused by the oxidation of flavonoids, particularly proanthocyanidins, which are polymers of flavan-3-ol subunits such as epicatechin and catechin. The tt10 mutant seeds accumulate more epicatechin monomers and more soluble proanthocyanidins than wild-type seeds. Moreover, intact testa cells of tt10 cannot trigger H2O2-independent browning in the presence of epicatechin and catechin, in contrast with wild-type cells. UV-visible light detection and mass spectrometry revealed that the major oxidation products obtained with epicatechin alone are yellow dimers called dehydrodiepicatechin A. These products differ from proanthocyanidins in the nature and position of their interflavan linkages. Flavonol composition was also affected in tt10 seeds, which exhibited a higher ratio of quercetin rhamnoside monomers versus dimers than wild-type seeds. We identified the TT10 gene by a candidate gene approach. TT10 encodes a protein with strong similarity to laccase-like polyphenol oxidases. It is expressed essentially in developing testa, where it colocalizes with the flavonoid end products proanthocyanidins and flavonols. Together, these data establish that TT10 is involved in the oxidative polymerization of flavonoids and functions as a laccase-type flavonoid oxidase. |
| 巻・号 | 17(11) |
| ページ | 2966-80 |
| 公開日 | 2005-11-1 |
| DOI | 10.1105/tpc.105.035154 |
| PII | tpc.105.035154 |
| PMID | 16243908 |
| PMC | PMC1276023 |
| MeSH | Amino Acid Sequence Arabidopsis / enzymology* Arabidopsis / genetics Arabidopsis / growth & development* Arabidopsis Proteins / genetics Arabidopsis Proteins / isolation & purification Arabidopsis Proteins / metabolism* Base Sequence Catechol Oxidase / genetics Catechol Oxidase / isolation & purification Catechol Oxidase / metabolism DNA, Complementary / analysis DNA, Complementary / genetics Flavonoids / metabolism* Gene Expression Regulation, Enzymologic / physiology Gene Expression Regulation, Plant / physiology Laccase / genetics Laccase / isolation & purification Laccase / metabolism* Molecular Sequence Data Mutation / genetics Oxidation-Reduction Oxidoreductases / genetics Oxidoreductases / isolation & purification Oxidoreductases / metabolism* Polymers / metabolism Promoter Regions, Genetic / genetics Seeds / enzymology* Seeds / genetics |
| IF | 9.618 |
| 引用数 | 241 |
| WOS 分野 | PLANT SCIENCES BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 10 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 3.0 |
| リソース情報 | |
| シロイヌナズナ / 植物培養細胞・遺伝子 | pda12625 |