| RRC ID |
61416
|
| 著者 |
Yasuda SY, Ikeda T, Shahsavarani H, Yoshida N, Nayer B, Hino M, Vartak-Sharma N, Suemori H, Hasegawa K.
|
| タイトル |
Chemically defined and growth-factor-free culture system for the expansion and derivation of human pluripotent stem cells.
|
| ジャーナル |
Nat Biomed Eng
|
| Abstract |
The large-scale and cost-effective production of quality-controlled human pluripotent stem cells (hPSCs) for use in cell therapy and drug discovery would ideally require a chemically defined xenobiotic-free culture system. Towards the development of such a system, costs associated with the use of recombinant proteins as supplements in basal culture media need to be reduced. Here, we describe a growth-factor-free culture medium that uses just three chemical compounds and a lower number of recombinant proteins than used in commercially available media. We show that the culture medium supports the long-term propagation of hPSCs, as confirmed by karyotype, the expression of pluripotency markers and the capacity to differentiate into cell types derived from the three embryonic germ layers. hPSCs growing in the medium were less dependent on glycolytic pathways than cells grown in medium containing growth factors. Moreover, the medium supported the generation of induced pluripotent stem cells derived from either human dermal fibroblasts or peripheral blood mononuclear cells. Our findings should facilitate the ongoing development of a completely xeno-free, chemically defined, synthetic culture system for hPSCs.
|
| 巻・号 |
2(3)
|
| ページ |
173-182
|
| 公開日 |
2018-3-1
|
| DOI |
10.1038/s41551-018-0200-7
|
| PII |
10.1038/s41551-018-0200-7
|
| PMID |
31015717
|
| MeSH |
Cell Culture Techniques / methods*
Cell Differentiation
Cells, Cultured
Culture Media / chemistry
Culture Media / metabolism
Fibroblasts / cytology
Fibroblasts / physiology
Humans
Intercellular Signaling Peptides and Proteins
Leukocytes, Mononuclear / cytology
Leukocytes, Mononuclear / physiology
Pluripotent Stem Cells* / cytology
Pluripotent Stem Cells* / physiology
|
| IF |
18.952
|
| リソース情報 |
| ヒト・動物細胞 |
253G1(HPS0002) |
