RRC ID 61463
Author Galal N, El Beialy W, Deyama Y, Yoshimura Y, Yoshikawa T, Suzuki K, Totsuka Y.
Title Effect of estrogen on bone resorption and inflammation in the temporomandibular joint cellular elements.
Journal Int J Mol Med
Abstract Several epidemiological studies have reported that temporomandibular disorder is more prevalent in women, which suggests the involvement of sex hormones, such as estrogen, in the pathogenesis of this disease. PCR amplification and Western blotting were employed to target the expression of estrogen receptors (ERs) in human fibroblast-like synovial and ATDC5 cells. The effect of estrogen was investigated through the expression of RANKL, osteoprotegerin (OPG), M-CSF/CSF-1 and c-fms. We showed expression of M-CSF/ CSF-1 and c-fms, with time-dependent increase in both after the addition of estrogen. Based on previous studies reporting that M-CSF/CSF-1 regulates the proliferation and differentiation of hemopoietic progenitor cells into mature macrophages, we put forward a new hypothesis based on the increased inflammation and tendency of females to suffer more from temporomandibular disorder (TMD) in the presence of external exacerbating factors. Detection of RANKL and OPG in ATDC5 and expression of both in HFLS was confirmed with complete disappearance of the RANKL band, and marked increase in the expression of OPG after 1 h from the addition of estrogen.
Volume 21(6)
Pages 785-90
Published 2008-6-1
PMID 18506373
MeSH Aged Animals Blotting, Western Bone Resorption / genetics Bone Resorption / metabolism Bone Resorption / prevention & control Cell Differentiation / drug effects Cell Line, Tumor Cells, Cultured Estrogens / pharmacology* Female Fibroblasts / cytology Fibroblasts / drug effects* Fibroblasts / metabolism Gene Expression / drug effects Gonadal Steroid Hormones / pharmacology Humans Inflammation / genetics Inflammation / metabolism Inflammation / prevention & control Macrophage Colony-Stimulating Factor / genetics Macrophage Colony-Stimulating Factor / metabolism Osteoprotegerin / genetics Osteoprotegerin / metabolism RANK Ligand / genetics RANK Ligand / metabolism RNA, Messenger / genetics RNA, Messenger / metabolism Receptor Activator of Nuclear Factor-kappa B / analysis Receptor Activator of Nuclear Factor-kappa B / drug effects Receptor Activator of Nuclear Factor-kappa B / genetics Receptor, Macrophage Colony-Stimulating Factor / genetics Receptor, Macrophage Colony-Stimulating Factor / metabolism Receptors, Estrogen / genetics Receptors, Estrogen / metabolism Reverse Transcriptase Polymerase Chain Reaction Synovial Membrane / cytology Synovial Membrane / drug effects* Synovial Membrane / metabolism Temporomandibular Joint / drug effects Temporomandibular Joint / metabolism Temporomandibular Joint / pathology Temporomandibular Joint Disorders / genetics Temporomandibular Joint Disorders / metabolism Temporomandibular Joint Disorders / prevention & control
IF 3.098
Human and Animal Cells ATDC5(RCB0565)