論文 - 詳細
| RRC ID | 61474 |
|---|---|
| 著者 | Nishimura K, Matsumoto R, Yonezawa Y, Nakagawa H. |
| タイトル | Effect of quercetin on cell protection via erythropoietin and cell injury of HepG2 cells. |
| ジャーナル | Arch Biochem Biophys |
| Abstract |
Quercetin is a flavonoid that has roles in both cytoprotection and cytotoxicity. The relation of queretin's cytoprotective and cytotoxic effects are unknown. Quercetin has been shown to induce expression of hypoxia-inducible factor, a protein that is known to regulate transcription of the erythropoietin (EPO) gene, and EPO is known to have a cytoprotective effect. This study used HepG2 cells to assess whether the cell-protective and/or cytotoxic roles of quercetin are mediated by promotion of EPO production. Increases in the levels of HIF-1α protein and EPO mRNA were quercetin concentration-dependent, with significant increases observed from 10 μM quercetin. Silencing of EPO expression by si-EPO RNA attenuated quercetin-induced cytoprotection against hydrogen peroxide toxicity. Cytotoxicity, evidenced by the induction of apoptosis, was significantly increased by exposure to 50 μM quercetin. Specifically, the levels of cleaved caspase-3 and Bax and the rate of cell death increased, and the level of Bcl-2 decreased, in cells treated with 50 μM quercetin. In contrast, exposure to 10 μM quercetin attenuated cisplatin-induced apoptosis. However, quercetin's ability to protect cells from cisplatin-induced apoptosis was eliminated when EPO expression was silenced using si-EPO RNA. Together, these results suggested that quercetin's cytoprotective effects in HepG2 cells are mediated via EPO production. |
| 巻・号 | 636 |
| ページ | 11-16 |
| 公開日 | 2017-12-15 |
| DOI | 10.1016/j.abb.2017.10.013 |
| PII | S0003-9861(17)30611-2 |
| PMID | 29080630 |
| MeSH | Caspase 3 / biosynthesis Cytoprotection / drug effects* Cytotoxins / pharmacology Erythropoietin / biosynthesis* Hep G2 Cells Humans Hydrogen Peroxide / metabolism* Proto-Oncogene Proteins c-bcl-2 / biosynthesis Quercetin / pharmacology* bcl-2-Associated X Protein / biosynthesis |
| IF | 3.391 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | Hep G2 |