論文 - 詳細
| RRC ID | 61496 |
|---|---|
| 著者 | Ohba T, Ishisaka M, Tsujii S, Tsuruma K, Shimazawa M, Kubo K, Umigai N, Iwawaki T, Hara H. |
| タイトル | Crocetin protects ultraviolet A-induced oxidative stress and cell death in skin in vitro and in vivo. |
| ジャーナル | Eur J Pharmacol |
| Abstract |
Crocetin, the aglycone of crocin, is a carotenoid found in fruits of gardenia (Gardeina jasminoides Ellis) and saffron (Crocus sativus L.). We investigated the protective effects of crocetin against ultraviolet-A (UV-A)-induced skin damage and explored the underlying mechanism. Human skin-derived fibroblasts cells (NB1-RGB) were damaged by exposure to UV-A irradiation (10J/cm(2)). Crocetin protected these cells against cell death and reduced the production of reactive oxygen species induced by UV-A irradiation. Crocetin treatment also suppressed induction of caspase-3 activation by UV-A irradiation. The effects of crocetin against oxidative stress were also examined by imaging of Keap1-dependent oxidative stress detector (OKD) mice. UV-A irradiation upregulated oxidative stress in the OKD mice skin, while crocetin administration (100mg/kg, p.o.) ameliorated this oxidative stress. Crocetin administration also decreased lipid peroxidation in the skin. These findings suggest that crocetin its observed protective effects against UV-A induced skin damage by reducing reactive oxygen species production and cell apoptosis. |
| 巻・号 | 789 |
| ページ | 244-253 |
| 公開日 | 2016-10-15 |
| DOI | 10.1016/j.ejphar.2016.07.036 |
| PII | S0014-2999(16)30472-1 |
| PMID | 27452919 |
| MeSH | Animals Antioxidants / pharmacology* Autophagy / drug effects Autophagy / radiation effects Carotenoids / pharmacology* Cell Death / drug effects Cell Death / radiation effects Crocus / chemistry Endoplasmic Reticulum Stress / drug effects Endoplasmic Reticulum Stress / radiation effects Lipid Peroxidation / drug effects Lipid Peroxidation / radiation effects Male Mice Oxidative Stress / drug effects* Oxidative Stress / radiation effects* Radiation-Protective Agents / pharmacology* Reactive Oxygen Species / metabolism Skin / cytology* Skin / drug effects Skin / metabolism Skin / radiation effects Ultraviolet Rays / adverse effects* Vitamin A / analogs & derivatives |
| IF | 3.263 |
| リソース情報 | |
| ヒト・動物細胞 | NB1RGB(RCB0222) |