RRC ID |
61507
|
著者 |
Akino T, Hida K, Hida Y, Tsuchiya K, Freedman D, Muraki C, Ohga N, Matsuda K, Akiyama K, Harabayashi T, Shinohara N, Nonomura K, Klagsbrun M, Shindoh M.
|
タイトル |
Cytogenetic abnormalities of tumor-associated endothelial cells in human malignant tumors.
|
ジャーナル |
Am J Pathol
|
Abstract |
Tumor blood vessels are thought to contain genetically normal and stable endothelial cells (ECs), unlike tumor cells, which typically display genetic instability. Yet, chromosomal aberration in human tumor-associated ECs (hTECs) in carcinoma has not yet been investigated. Here we isolated TECs from 20 human renal cell carcinomas and analyzed their cytogenetic abnormalities. The degree of aneuploidy was analyzed by fluorescence in situ hybridization using chromosome 7 and chromosome 8 DNA probes in isolated hTECs. In human renal cell carcinomas, 22-58% (median, 33%) of uncultured hTECs were aneuploid, whereas normal ECs were diploid. The mechanisms governing TEC aneuploidy were then studied using mouse TECs (mTECs) isolated from xenografts of human epithelial tumors. To investigate the contribution of progenitor cells to aneuploidy in mTECs, CD133(+) and CD133(-) mTECs were compared for aneuploidy. CD133(+) mTECs showed aneuploidy more frequently than CD133(-) mTECs. This is the first report showing cytogenetic abnormality of hTECs in carcinoma, contrary to traditional belief. Cytogenetic alterations in tumor vessels of carcinoma therefore can occur and may play a significant role in modifying tumor- stromal interactions.
|
巻・号 |
175(6)
|
ページ |
2657-67
|
公開日 |
2009-12-1
|
DOI |
10.2353/ajpath.2009.090202
|
PII |
S0002-9440(10)60773-0
|
PMID |
19875502
|
PMC |
PMC2789618
|
MeSH |
AC133 Antigen
Antigens, CD / biosynthesis
Carcinoma, Renal Cell / blood supply*
Carcinoma, Renal Cell / genetics*
Cell Separation
Chromosomal Proteins, Non-Histone / biosynthesis
Chromosomal Proteins, Non-Histone / genetics
Chromosome Aberrations
Endothelial Cells / pathology*
Flow Cytometry
Glycoproteins / biosynthesis
Humans
Immunohistochemistry
In Situ Hybridization, Fluorescence
Kidney Neoplasms / blood supply*
Kidney Neoplasms / genetics*
Neovascularization, Pathologic / genetics*
Peptides
Reverse Transcriptase Polymerase Chain Reaction
|
IF |
3.491
|
リソース情報 |
ヒト・動物細胞 |
OS-RC-2(RCB0735) |