RRC ID 61507
著者 Akino T, Hida K, Hida Y, Tsuchiya K, Freedman D, Muraki C, Ohga N, Matsuda K, Akiyama K, Harabayashi T, Shinohara N, Nonomura K, Klagsbrun M, Shindoh M.
タイトル Cytogenetic abnormalities of tumor-associated endothelial cells in human malignant tumors.
ジャーナル Am J Pathol
Abstract Tumor blood vessels are thought to contain genetically normal and stable endothelial cells (ECs), unlike tumor cells, which typically display genetic instability. Yet, chromosomal aberration in human tumor-associated ECs (hTECs) in carcinoma has not yet been investigated. Here we isolated TECs from 20 human renal cell carcinomas and analyzed their cytogenetic abnormalities. The degree of aneuploidy was analyzed by fluorescence in situ hybridization using chromosome 7 and chromosome 8 DNA probes in isolated hTECs. In human renal cell carcinomas, 22-58% (median, 33%) of uncultured hTECs were aneuploid, whereas normal ECs were diploid. The mechanisms governing TEC aneuploidy were then studied using mouse TECs (mTECs) isolated from xenografts of human epithelial tumors. To investigate the contribution of progenitor cells to aneuploidy in mTECs, CD133(+) and CD133(-) mTECs were compared for aneuploidy. CD133(+) mTECs showed aneuploidy more frequently than CD133(-) mTECs. This is the first report showing cytogenetic abnormality of hTECs in carcinoma, contrary to traditional belief. Cytogenetic alterations in tumor vessels of carcinoma therefore can occur and may play a significant role in modifying tumor- stromal interactions.
巻・号 175(6)
ページ 2657-67
公開日 2009-12-1
DOI 10.2353/ajpath.2009.090202
PII S0002-9440(10)60773-0
PMID 19875502
PMC PMC2789618
MeSH AC133 Antigen Antigens, CD / biosynthesis Carcinoma, Renal Cell / blood supply* Carcinoma, Renal Cell / genetics* Cell Separation Chromosomal Proteins, Non-Histone / biosynthesis Chromosomal Proteins, Non-Histone / genetics Chromosome Aberrations Endothelial Cells / pathology* Flow Cytometry Glycoproteins / biosynthesis Humans Immunohistochemistry In Situ Hybridization, Fluorescence Kidney Neoplasms / blood supply* Kidney Neoplasms / genetics* Neovascularization, Pathologic / genetics* Peptides Reverse Transcriptase Polymerase Chain Reaction
IF 3.491
リソース情報
ヒト・動物細胞 OS-RC-2(RCB0735)