| RRC ID |
61529
|
| 著者 |
Haniu H, Saito N, Matsuda Y, Kim YA, Park KC, Tsukahara T, Usui Y, Aoki K, Shimizu M, Ogihara N, Hara K, Takanashi S, Okamoto M, Ishigaki N, Nakamura K, Kato H.
|
| タイトル |
Elucidation mechanism of different biological responses to multi-walled carbon nanotubes using four cell lines.
|
| ジャーナル |
Int J Nanomedicine
|
| Abstract |
We examined differences in cellular responses to multi-walled carbon nanotubes (MWCNTs) using malignant pleural mesothelioma cells (MESO-1), bronchial epithelial cells (BEAS-2B), neuroblastoma cells (IMR-32), and monoblastic cells (THP-1), before and after differentiation. MESO-1, BEAS-2B and differentiated THP-1 cells actively endocytosed MWCNTs, resulting in cytotoxicity with lysosomal injury. However, cytotoxicity did not occur in IMR-32 or undifferentiated THP-1 cells. Both differentiated and undifferentiated THP-1 cells exhibited an inflammatory response. Carbon blacks were endocytosed by the same cell types without lysosomal damage and caused cytokine secretion, but they did not cause cytotoxicity. These results indicate that the cytotoxicity of MWCNTs requires not only cellular uptake but also lysosomal injury. Furthermore, it seems that membrane permeability or cytokine secretion without cytotoxicity results from several active mechanisms. Clarification of the cellular recognition mechanism for MWCNTs is important for developing safer MWCNTs.
|
| 巻・号 |
6
|
| ページ |
3487-97
|
| 公開日 |
2011-1-1
|
| DOI |
10.2147/IJN.S26689
|
| PII |
ijn-6-3487
|
| PMID |
22267932
|
| PMC |
PMC3260041
|
| MeSH |
Cell Line, Transformed
Cell Line, Tumor
Cell Survival / drug effects
Cytokines / metabolism
Endocytosis
Humans
Nanotubes, Carbon / chemistry*
Nanotubes, Carbon / toxicity
Reactive Oxygen Species / metabolism
|
| IF |
5.115
|
| リソース情報 |
| ヒト・動物細胞 |
ACC-MESO-1(RCB2292)
THP-1(RCB1189) |
