RRC ID 61553
Author Hayashida Y, Nakata K, Ishikawa H, Taniguchi K, Migita K, Nakao K, Kato Y, Eguchi K.
Title Epidermal growth factor-mediated cell maturation and apoptosis in RGM-1 rat gastric mucosal cells.
Journal Int J Mol Med
Abstract Epidermal growth factor (EGF) and heparin-binding EGF-like growth factor (HB-EGF) exert their activities through the same cell surface receptor. In the present study, the role of EGF in cell kinetics of rat gastric epithelial cell line, RGM-1, in which HB-EGF is known to function in an autocrine fashion, was investigated. EGF stimulated the mucus glycoprotein synthesis which is characterized with cell maturation, but competed with HB-EGF for cell proliferation. By flow cytometric analysis, EGF inhibited G2/M transition by 48 h after stimulation and induced apoptosis manifested by hypodiploid DNA by 72 h after stimulation. Consistent with this, DNA ladder formation together with the histone-associated DNA fragments was found in EGF-treated cells. These results indicate that EGF and HB-EGF possess different functions in RGM-1 cells and that EGF acts as a mediator of both cell maturation and apoptosis in these cells.
Volume 4(3)
Pages 253-6
Published 1999-9
DOI 10.3892/ijmm.4.3.253
PMID 10425274
MeSH Animals Apoptosis / drug effects* Cell Cycle / drug effects Cell Division / drug effects Cell Line Cell Survival / drug effects Epidermal Growth Factor / metabolism Epidermal Growth Factor / pharmacology* Flow Cytometry Gastric Mucosa / drug effects* Gastric Mucosa / metabolism Glucosamine / metabolism Glycoproteins / biosynthesis Heparin-binding EGF-like Growth Factor Histones / metabolism Intercellular Signaling Peptides and Proteins Ploidies Rats Rats, Wistar
Human and Animal Cells RGM1(RCB0876)