論文 - 詳細
| RRC ID | 6158 |
|---|---|
| 著者 | Fujimoto M, Arimura S, Ueda T, Takanashi H, Hayashi Y, Nakano A, Tsutsumi N. |
| タイトル | Arabidopsis dynamin-related proteins DRP2B and DRP1A participate together in clathrin-coated vesicle formation during endocytosis. |
| ジャーナル | Proc Natl Acad Sci U S A |
| Abstract |
Endocytosis performs a wide range of functions in animals and plants. Clathrin-coated vesicle (CCV) formation is an initial step of endocytosis, and in animal cells is largely achieved by dynamins. However, little is known of its molecular mechanisms in plant cells. To identify dynamin-related proteins (DRPs) involved in endocytic CCV formation in plant cells, we compared the behaviors of two structurally different Arabidopsis DRPs, DRP2B and DRP1A, with those of the clathrin light chain (CLC), a marker of CCVs, at the plasma membrane by variable incidence angle fluorescent microscopy (VIAFM). DRP2B shares domain organization with animal dynamins whereas DRP1A is plant-specific. We show that green fluorescent protein (GFP)-tagged DRP2B and DRP1A colocalized with CLC tagged with monomeric Kusabira Orange (mKO) in Arabidopsis cultured cells. Time-lapse VIAFM observations suggested that both GFP-DRP2B and GFP-DRP1A appeared and accumulated on the existing mKO-CLC foci and disappeared at the same time as or immediately after the disappearance of mKO-CLC. Moreover, DRP2B and DRP1A colocalized and assembled/disassembled together at the plasma membrane in Arabidopsis cells. A yeast two-hybrid assay showed that DRP2B and DRP1A interacted with each other. An inhibitor of clathrin-mediated endocytosis, tyrphostin A23, disturbed the localization of DRP1A, but had little effect on the localization of DRP2B, indicating that DRP1A and DRP2B have different molecular properties. These results suggest that DRP2B and DRP1A participate together in endocytic CCV formation in Arabidopsis cells despite the difference of their molecular properties. |
| 巻・号 | 107(13) |
| ページ | 6094-9 |
| 公開日 | 2010-3-30 |
| DOI | 10.1073/pnas.0913562107 |
| PII | 0913562107 |
| PMID | 20231465 |
| PMC | PMC2851877 |
| MeSH | Arabidopsis / drug effects Arabidopsis / genetics Arabidopsis / metabolism* Arabidopsis Proteins / chemistry Arabidopsis Proteins / genetics Arabidopsis Proteins / metabolism* Base Sequence Binding Sites Cell Membrane / metabolism Clathrin Light Chains / metabolism Clathrin-Coated Vesicles / drug effects Clathrin-Coated Vesicles / metabolism* DNA Primers / genetics DNA, Plant / genetics Dynamins / chemistry Dynamins / genetics Dynamins / metabolism* Endocytosis / drug effects Endocytosis / physiology GTP-Binding Proteins / chemistry GTP-Binding Proteins / genetics GTP-Binding Proteins / metabolism* Green Fluorescent Proteins / genetics Green Fluorescent Proteins / metabolism Microscopy, Fluorescence Models, Biological Molecular Sequence Data Plants, Genetically Modified Protein Structure, Tertiary Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / metabolism Two-Hybrid System Techniques Tyrphostins / pharmacology |
| IF | 9.412 |
| 引用数 | 89 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | F1000Research |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| シロイヌナズナ / 植物培養細胞・遺伝子 | pda07543 |