論文 - 詳細
| RRC ID | 61605 |
|---|---|
| 著者 | Hou A, Tong L. |
| タイトル | Expression, Regulation, and Effects of Interleukin-17f in the Human Ocular Surface. |
| ジャーナル | Ocul Immunol Inflamm |
| Abstract |
PURPOSE:To evaluate the basal and possibly stimulated expression of interleukin (IL)-17 in the context of the ocular surface and potential downstream effects. METHODS:Western blot and immunofluorescent staining were used to evaluate IL-17F expression in human cornea/conjunctival tissues and cornea epithelial cell line. Cytokines and matrix metalloproteinase (MMP) transcripts were quantified by qPCR. IL-17F effects on NF-κB were investigated by the secretary alkaline phosphatase assay. RESULTS:IL-17F was expressed in the cytoplasm of human corneal and conjunctival epithelial tissues. In the corneal cell line, exogenous IL-17F did not increase the NF-κB activity, but Pam3CSK4 increased IL-17F transcripts. IL-17F stimulated MMP-9 activity, promoted IL-6, IL-8, tumor necrosis factor-α transcripts levels, and depressed monocyte chemoattractant protein-1 expression, but did not affect transforming growth factor beta-1 transcript levels. CONCLUSIONS:Normal corneal/conjunctival epithelial cells express IL-17F. Microbial agents may stimulate IL-17F via the NF-κB pathway. Matrix dissolution stimulated by IL-17F may have a role in the melting and necrosis of cornea in severe inflammation. |
| 巻・号 | 26(7) |
| ページ | 1069-1077 |
| 公開日 | 2018-1-1 |
| DOI | 10.1080/09273948.2017.1316411 |
| PMID | 28537453 |
| MeSH | Blotting, Western Cell Line Conjunctiva / drug effects Conjunctiva / metabolism* Cornea / drug effects Cornea / metabolism* Cytokines / genetics Epithelium, Corneal / drug effects Epithelium, Corneal / metabolism Fluorescent Antibody Technique, Indirect Gene Expression Regulation / physiology* Humans Interleukin-17 / genetics* Interleukin-17 / pharmacology* Matrix Metalloproteinases / genetics NF-kappa B / metabolism Real-Time Polymerase Chain Reaction |
| IF | 2.112 |
| リソース情報 | |
| ヒト・動物細胞 | HCE-T(RCB2280) |