Reference - Detail
RRC ID | 61605 |
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Author | Hou A, Tong L. |
Title | Expression, Regulation, and Effects of Interleukin-17f in the Human Ocular Surface. |
Journal | Ocul Immunol Inflamm |
Abstract |
PURPOSE:To evaluate the basal and possibly stimulated expression of interleukin (IL)-17 in the context of the ocular surface and potential downstream effects. METHODS:Western blot and immunofluorescent staining were used to evaluate IL-17F expression in human cornea/conjunctival tissues and cornea epithelial cell line. Cytokines and matrix metalloproteinase (MMP) transcripts were quantified by qPCR. IL-17F effects on NF-κB were investigated by the secretary alkaline phosphatase assay. RESULTS:IL-17F was expressed in the cytoplasm of human corneal and conjunctival epithelial tissues. In the corneal cell line, exogenous IL-17F did not increase the NF-κB activity, but Pam3CSK4 increased IL-17F transcripts. IL-17F stimulated MMP-9 activity, promoted IL-6, IL-8, tumor necrosis factor-α transcripts levels, and depressed monocyte chemoattractant protein-1 expression, but did not affect transforming growth factor beta-1 transcript levels. CONCLUSIONS:Normal corneal/conjunctival epithelial cells express IL-17F. Microbial agents may stimulate IL-17F via the NF-κB pathway. Matrix dissolution stimulated by IL-17F may have a role in the melting and necrosis of cornea in severe inflammation. |
Volume | 26(7) |
Pages | 1069-1077 |
Published | 2018-1-1 |
DOI | 10.1080/09273948.2017.1316411 |
PMID | 28537453 |
MeSH | Blotting, Western Cell Line Conjunctiva / drug effects Conjunctiva / metabolism* Cornea / drug effects Cornea / metabolism* Cytokines / genetics Epithelium, Corneal / drug effects Epithelium, Corneal / metabolism Fluorescent Antibody Technique, Indirect Gene Expression Regulation / physiology* Humans Interleukin-17 / genetics* Interleukin-17 / pharmacology* Matrix Metalloproteinases / genetics NF-kappa B / metabolism Real-Time Polymerase Chain Reaction |
IF | 2.112 |
Resource | |
Human and Animal Cells | HCE-T(RCB2280) |