RRC ID 61666
著者 Kinoshita Y, Nakata H, Hassan S, Asahara M, Kawanami C, Matsushima Y, Naribayashi-Inomoto Y, Ping CY, Min D, Nakamura A.
タイトル Gene expression of keratinocyte and hepatocyte growth factors during the healing of rat gastric mucosal lesions.
ジャーナル Gastroenterology
Abstract BACKGROUND & AIMS:The factors that stimulate the growth of gastric mucosal cells during gastric mucosal healing are not completely understood. This study was designed to investigate the gene expression of keratinocyte growth factor (KGF) and hepatocyte growth factor (HGF) in the healing of gastric mucosal lesions.
METHODS:Northern blot analysis and reverse-transcription polymerase chain reaction were used to show HGF and KGF messenger RNA.
RESULTS:Transcripts of HGF and KGF were not shown in rat gastric mucosal epithelium but were found in submucosal and muscular layers under normal conditions. When acute gastric mucosal lesions were induced by indomethacin treatment, expression of HGF messenger RNA was augmented in submucosal, muscular, or serosal layers, whereas the transcript of KGF was not influenced. When rat gastric mucosal epithelial cell line RGM1 and rat gastric mucosal primary culture cells were incubated with HGF or KGF, their proliferation was enhanced.
CONCLUSIONS:The results showed increased gene expression of HGF together with constant production of KGF during gastric mucosal healing.
巻・号 109(4)
ページ 1068-77
公開日 1995-10-1
DOI 10.1016/0016-5085(95)90564-2
PII S0016508595003167
PMID 7557071
MeSH Acetates / pharmacology Acetic Acid Animals Base Sequence Cell Line Cells, Cultured DNA / biosynthesis Fibroblast Growth Factor 10 Fibroblast Growth Factor 7 Fibroblast Growth Factors* Gastric Mucosa / chemistry Gastric Mucosa / physiology* Gene Expression Growth Substances / genetics* Hepatocyte Growth Factor / genetics* In Situ Hybridization Indomethacin / pharmacology Keratinocytes / physiology* Male Molecular Sequence Data Polymerase Chain Reaction RNA, Messenger / analysis Rats Rats, Sprague-Dawley Receptor, Fibroblast Growth Factor, Type 2 Receptors, Fibroblast Growth Factor* Receptors, Growth Factor / analysis Stomach Ulcer / metabolism
IF 17.373
リソース情報
ヒト・動物細胞 RGM1(RCB0876)