RRC ID 61701
Author Hunziker J, Nishida K, Kondo A, Kishimoto S, Ariizumi T, Ezura H.
Title Multiple gene substitution by Target-AID base-editing technology in tomato.
Journal Sci Rep
Abstract The use of Target activation-induced cytidine deaminase (Target-AID) base-editing technology with the CRISPR-Cas 9 system fused with activation-induced cytidine deaminase (AID) resulted in the substitution of a cytidine with a thymine. In previous experiments focusing on a single target gene, this system has been reported to work in several plant species, including tomato (Solanum lycopersicum L.). In this research, we used Target-AID technology to target multiple genes related to carotenoid accumulation in tomato. We selected 3 genes, SlDDB1, SlDET1 and SlCYC-B, for their roles in carotenoid accumulation. Among 12 edited T0 lines, we obtained 10 independent T0 lines carrying nucleotide substitutions in the three targeted genes, with several allelic versions for each targeted gene. The two edited lines showed significant differences in carotenoid accumulation. These results demonstrate that Target-AID technology is a highly efficient tool for targeting multiple genes with several allelic versions.
Volume 10(1)
Pages 20471
Published 2020-11-24
DOI 10.1038/s41598-020-77379-2
PII 10.1038/s41598-020-77379-2
PMID 33235312
PMC PMC7686336
MeSH Alleles Base Pairing CRISPR-Cas Systems Carotenoids / metabolism Cytidine Deaminase / metabolism* Gene Editing / methods* Plant Proteins / genetics* Solanum lycopersicum / genetics Solanum lycopersicum / growth & development* Solanum lycopersicum / metabolism
IF 3.998
Tomato TOMJPF00001 (Micro-Tom)