論文 - 詳細
| RRC ID | 61726 |
|---|---|
| 著者 | Chiliveru S, Birkelund S, Paludan SR. |
| タイトル | Induction of interferon-stimulated genes by Chlamydia pneumoniae in fibroblasts is mediated by intracellular nucleotide-sensing receptors. |
| ジャーナル | PLoS One |
| Abstract |
BACKGROUND:Recognition of microorganisms by the innate immune system is mediated by pattern recognition receptors, including Toll-like receptors and cytoplasmic RIG-I-like receptors. Chlamydia, which include several human pathogenic species, are obligate intracellular gram-negative bacteria that replicate in cytoplasmic vacuoles. The infection triggers a host response contributing to both bacterial clearance and tissue damage. For instance, type I interferons (IFN)s have been demonstrated to exacerbate the course of Chlamydial lung infections in mice. METHODS/PRINCIPAL FINDINGS:Here we show that Chlamydia pneumoniae induces expression of IFN-stimulated genes (ISG)s dependent on recognition by nucleotide-sensing Toll-like receptors and RIG-I-like receptors, localized in endosomes and the cytoplasm, respectively. The ISG response was induced with a delayed kinetics, compared to virus infections, and was dependent on bacterial replication and the bacterial type III secretion system (T3SS). CONCLUSIONS/SIGNIFICANCE:Activation of the IFN response during C. pneumoniae infection is mediated by intracellular nucleotide-sensing PRRs, which operate through a mechanism dependent on the bacterial T3SS. Strategies to inhibit the chlamydial T3SS may be used to limit the detrimental effects of the type I IFN system in the host response to Chlamydia infection. |
| 巻・号 | 5(4) |
| ページ | e10005 |
| 公開日 | 2010-4-6 |
| DOI | 10.1371/journal.pone.0010005 |
| PMID | 20386592 |
| PMC | PMC2850306 |
| MeSH | Animals Bacterial Proteins / metabolism Cells, Cultured Chlamydophila Infections / immunology Chlamydophila pneumoniae / immunology* Fibroblasts / microbiology* Genes / immunology Host-Pathogen Interactions / immunology Immunity, Innate* Interferons / genetics* Mice Mice, Inbred C57BL Mice, Knockout Nucleotides / metabolism Receptors, Pattern Recognition / immunology* Toll-Like Receptors Transcriptional Activation / immunology* |
| IF | 2.74 |
| リソース情報 | |
| ヒト・動物細胞 | Atg5^(-/-)MEF(RCB2711) |