RRC ID 6173
著者 Yamazaki T, Takata N, Uemura M, Kawamura Y.
タイトル Arabidopsis synaptotagmin SYT1, a type I signal-anchor protein, requires tandem C2 domains for delivery to the plasma membrane.
ジャーナル J Biol Chem
Abstract The correct localization of integral membrane proteins to subcellular compartments is important for their functions. Synaptotagmin contains a single transmembrane domain that functions as a type I signal-anchor sequence in its N terminus and two calcium-binding domains (C(2)A and C(2)B) in its C terminus. Here, we demonstrate that the localization of an Arabidopsis synaptotagmin homolog, SYT1, to the plasma membrane (PM) is modulated by tandem C2 domains. An analysis of the roots of a transformant-expressing green fluorescent protein-tagged SYT1 driven by native SYT1 promoter suggested that SYT1 is synthesized in the endoplasmic reticulum, and then delivered to the PM via the exocytotic pathway. We transiently expressed a series of truncated proteins in protoplasts, and determined that tandem C(2)A-C(2)B domains were necessary for the localization of SYT1 to the PM. The PM localization of SYT1 was greatly reduced following mutation of the calcium-binding motifs of the C(2)B domain, based on sequence comparisons with other homologs, such as endomembrane-localized SYT5. The localization of SYT1 to the PM may have been required for the functional divergence that occurred in the molecular evolution of plant synaptotagmins.
巻・号 285(30)
ページ 23165-76
公開日 2010-7-23
DOI 10.1074/jbc.M109.084046
PII S0021-9258(20)60359-3
PMID 20498364
PMC PMC2906310
MeSH Amino Acid Motifs Amino Acid Sequence Arabidopsis / cytology* Arabidopsis / metabolism* Cell Membrane / metabolism* Endoplasmic Reticulum / metabolism Gene Deletion Models, Molecular Molecular Sequence Data Phylogeny Plant Leaves / cytology Plant Leaves / metabolism Plant Roots / cytology Plant Roots / metabolism Protein Structure, Tertiary Protein Transport Recombinant Fusion Proteins / chemistry Recombinant Fusion Proteins / classification Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / metabolism Synaptotagmin I / chemistry* Synaptotagmin I / classification Synaptotagmin I / genetics Synaptotagmin I / metabolism*
IF 4.238
引用数 27
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
シロイヌナズナ / 植物培養細胞・遺伝子 pda01369 pda09961