論文 - 詳細
| RRC ID | 6173 |
|---|---|
| 著者 | Yamazaki T, Takata N, Uemura M, Kawamura Y. |
| タイトル | Arabidopsis synaptotagmin SYT1, a type I signal-anchor protein, requires tandem C2 domains for delivery to the plasma membrane. |
| ジャーナル | J Biol Chem |
| Abstract |
The correct localization of integral membrane proteins to subcellular compartments is important for their functions. Synaptotagmin contains a single transmembrane domain that functions as a type I signal-anchor sequence in its N terminus and two calcium-binding domains (C(2)A and C(2)B) in its C terminus. Here, we demonstrate that the localization of an Arabidopsis synaptotagmin homolog, SYT1, to the plasma membrane (PM) is modulated by tandem C2 domains. An analysis of the roots of a transformant-expressing green fluorescent protein-tagged SYT1 driven by native SYT1 promoter suggested that SYT1 is synthesized in the endoplasmic reticulum, and then delivered to the PM via the exocytotic pathway. We transiently expressed a series of truncated proteins in protoplasts, and determined that tandem C(2)A-C(2)B domains were necessary for the localization of SYT1 to the PM. The PM localization of SYT1 was greatly reduced following mutation of the calcium-binding motifs of the C(2)B domain, based on sequence comparisons with other homologs, such as endomembrane-localized SYT5. The localization of SYT1 to the PM may have been required for the functional divergence that occurred in the molecular evolution of plant synaptotagmins. |
| 巻・号 | 285(30) |
| ページ | 23165-76 |
| 公開日 | 2010-7-23 |
| DOI | 10.1074/jbc.M109.084046 |
| PII | S0021-9258(20)60359-3 |
| PMID | 20498364 |
| PMC | PMC2906310 |
| MeSH | Amino Acid Motifs Amino Acid Sequence Arabidopsis / cytology* Arabidopsis / metabolism* Cell Membrane / metabolism* Endoplasmic Reticulum / metabolism Gene Deletion Models, Molecular Molecular Sequence Data Phylogeny Plant Leaves / cytology Plant Leaves / metabolism Plant Roots / cytology Plant Roots / metabolism Protein Structure, Tertiary Protein Transport Recombinant Fusion Proteins / chemistry Recombinant Fusion Proteins / classification Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / metabolism Synaptotagmin I / chemistry* Synaptotagmin I / classification Synaptotagmin I / genetics Synaptotagmin I / metabolism* |
| IF | 4.238 |
| 引用数 | 27 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| シロイヌナズナ / 植物培養細胞・遺伝子 | pda01369 pda09961 |