論文 - 詳細
| RRC ID | 61732 |
|---|---|
| 著者 | Ura K, Morimoto I, Watanabe K, Saito K, Yanagihara N, Eto S. |
| タイトル | Interleukin (IL)-4 and IL-13 inhibit the differentiation of murine osteoblastic MC3T3-E1 cells. |
| ジャーナル | Endocr J |
| Abstract |
Interleukin-4 (IL-4) inhibits the spontaneous and stimulated bone resorption resulting from the inhibition of osteoclast formation, as well as osteoclastic activity. Since IL-13 shares some biological properties with IL-4, it was recently reported that IL-13 inhibits bone resorption. The present study was designed to determine the effects of murine IL-4 (IL-4) and murine IL-13 (IL-13) on the murine osteoblastic cell line MC3T3-E1. IL-4 and IL-13 stimulated 3H-thymidine incorporation in the MC3T3-E1 cells and its proliferation in dose dependent manners. A spontaneous increase in alkaline phosphatase (ALP) activity in the cells after plating was inhibited by IL-4 or IL-13, and both cytokines blunted an increase in ALP activity by human parathyroid hormone (PTH) (1-34). PTH-stimulated cyclic AMP (cAMP) production was inhibited by pretreatment with IL-4 and IL-13 for 48 hr in dose dependent manners. Pretreatment with IL-4 and IL-13 for 48 hr caused a decrease in PTH-induced cAMP production at any stimulatory concentration. However, the effective dose (ED50) was unchanged by the pretreatment with these cytokines. Pretreatment with IL-4 and IL-13 did not modulate cAMP generation by forskolin. In contrast, cAMP generation by PGE2 is greater in the cells treated with the cytokines compared to those without the cytokines. These results indicate that IL-4 and IL-13 act on MC3T3-E1 cells in the same manner, stimulating cell proliferation, but inhibiting cell differentiation. The inhibition of osteoblast differentiation by IL-4 and IL-13 may be associated with a decrease in PTH actions on osteoblasts. |
| 巻・号 | 47(3) |
| ページ | 293-302 |
| 公開日 | 2000-6-1 |
| DOI | 10.1507/endocrj.47.293 |
| PMID | 11036873 |
| MeSH | Alkaline Phosphatase / metabolism Animals Cell Differentiation / drug effects Cells, Cultured Cyclic AMP / metabolism Dinoprostone / pharmacology Humans Interleukin-13 / pharmacology* Interleukin-3 / pharmacology* Mice Osteoblasts / drug effects* Osteoblasts / metabolism Parathyroid Hormone / pharmacology Peptide Fragments / pharmacology Thymidine / metabolism |
| IF | 1.952 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | MC3T3-E1(RCB1126) |