論文 - 詳細
| RRC ID | 61734 |
|---|---|
| 著者 | Makiishi-Shimobayashi C, Tsujimura T, Iwasaki T, Yamada N, Sugihara A, Okamura H, Hayashi S, Terada N. |
| タイトル | Interleukin-18 up-regulates osteoprotegerin expression in stromal/osteoblastic cells. |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
Osteoprotegerin (OPG) and osteoclast differentiation factor (ODF) are crucial regulators of osteoclastogenesis. To determine the biological role of interleukin (IL)-18 produced by stromal/osteoblastic cells in osteoclastogenesis, we examined the effects of IL-18 on the OPG and ODF mRNA levels in these cells. When bone marrow stromal ST2 cells, osteoblastic MC3T3-E1 cells, and mouse calvarial osteoblasts were stimulated with IL-18, the expression of OPG mRNA, but not ODF mRNA, was transiently increased, its expression reaching a maximal level at 3 h after the beginning of the culture. In accordance with this observation, all these cells expressed the mRNAs of two IL-18 receptor components and MyD88, an adapter molecule involved in IL-18 signaling. Moreover, in these cells, mitogen-activated protein kinase was phosphorylated after stimulation with IL-18. These results suggest that stromal/osteoblastic cells are IL-18-responsive cells and that IL-18 may inhibit osteoclastogenesis by up-regulating OPG expression, without stimulation of ODF production, in stromal/osteoblastic cells. |
| 巻・号 | 281(2) |
| ページ | 361-6 |
| 公開日 | 2001-2-23 |
| DOI | 10.1006/bbrc.2001.4380 |
| PII | S0006291X01943803 |
| PMID | 11181055 |
| MeSH | Adaptor Proteins, Signal Transducing Animals Animals, Newborn Antigens, Differentiation / genetics Blotting, Western Cell Line Dose-Response Relationship, Drug Gene Expression Regulation / drug effects Glycoproteins / genetics* Interleukin-18 / pharmacology* Interleukin-18 Receptor alpha Subunit Mice Mitogen-Activated Protein Kinases / drug effects Mitogen-Activated Protein Kinases / metabolism Myeloid Differentiation Factor 88 Osteoblasts / cytology Osteoblasts / drug effects* Osteoblasts / metabolism Osteoprotegerin Phosphorylation / drug effects RNA, Messenger / drug effects RNA, Messenger / genetics RNA, Messenger / metabolism Receptors, Cytoplasmic and Nuclear / genetics* Receptors, Immunologic* Receptors, Interleukin / genetics Receptors, Interleukin-18 Receptors, Tumor Necrosis Factor Reverse Transcriptase Polymerase Chain Reaction Stromal Cells / cytology Stromal Cells / drug effects* Stromal Cells / metabolism Up-Regulation / drug effects |
| IF | 2.985 |
| リソース情報 | |
| ヒト・動物細胞 | MC3T3-E1(RCB1126) |