RRC ID 61768
Author Yamada K, Itoh R.
Title Involvement of DNA polymerase delta and/or epsilon in joining UV-induced DNA single strand breaks in human fibroblasts (comparison of effects of butylphenyldeoxyguanosine with aphidicolin).
Journal Biochim Biophys Acta
Abstract DNA polymerases involved in ultraviolet (UV)-induced DNA repair were studied in human fibroblasts using the inhibitors of DNA polymerases, aphidicolin which inhibits DNA polymerases alpha, delta and epsilon, and butylphenyldeoxyguanosine (BuPGdR) which inhibits DNA polymerase alpha strongly and weakly inhibits delta and epsilon. Both inhibitors inhibited replicative DNA synthesis in a dose dependent manner as measured by thymidine incorporation. However, BuPGdR did not accumulate single strand breaks in cells irradiated with 5 J/m2 UV-light even at the highest dosage tested, indicating that BuPGdR does not inhibit DNA repair. On the other hand, aphidicolin accumulated single strand breaks in UV-light irradiated cells. These results suggest that DNA polymerase delta and/or epsilon are mainly involved in UV-induced DNA repair.
Volume 1219(2)
Pages 302-6
Published 1994-10-18
DOI 10.1016/0167-4781(94)90052-3
PII 0167-4781(94)90052-3
PMID 7918625
MeSH Aphidicolin / pharmacology DNA / biosynthesis DNA Polymerase II DNA Polymerase III DNA Repair* DNA Replication / drug effects DNA-Directed DNA Polymerase / metabolism* Deoxyguanosine / analogs & derivatives Deoxyguanosine / pharmacology Fibroblasts Humans In Vitro Techniques Nucleic Acid Synthesis Inhibitors Ultraviolet Rays
IF 3.411
Human and Animal Cells NB1RGB(RCB0222)