| RRC ID |
61770
|
| 著者 |
Furugen A, Ishiguro Y, Kobayashi M, Narumi K, Nishimura A, Hirano T, Iseki K.
|
| タイトル |
Involvement of l-type amino acid transporter 1 in the transport of gabapentin into human placental choriocarcinoma cells.
|
| ジャーナル |
Reprod Toxicol
|
| Abstract |
Gabapentin (GBP) is a widely used antiepileptic drug, with potential for use in the treatment of epilepsy in pregnant women. Although studies have examined GBP transport mechanisms across the blood-brain barrier, kidney, and intestine, the mechanism in the placenta has not been fully elucidated. We previously reported that GBP accumulates at high concentrations in human placental choriocarcinoma BeWo cells. The purpose of this study was to examine the transport mechanism of GBP in placental choriocarcinoma cells (BeWo and JEG-3), and to identify the carrier involved. High concentrations of intracellular GBP accumulations were also found in JEG-3 cells. A kinetic analysis showed that a single carrier system was involved in the uptake of GBP. Furthermore, substrates for l-type amino acid transporter (LAT) and siRNAs targeted to LAT1 significantly decreased GBP uptake. Our observations from this study suggest that LAT1 is the main contributor to GBP transport in placental choriocarcinoma cells.
|
| 巻・号 |
67
|
| ページ |
48-55
|
| 公開日 |
2017-1-1
|
| DOI |
10.1016/j.reprotox.2016.11.002
|
| PII |
S0890-6238(16)30392-6
|
| PMID |
27818298
|
| MeSH |
Amines / metabolism
Amines / pharmacokinetics*
Anticonvulsants / metabolism
Anticonvulsants / pharmacokinetics*
Biological Transport
Blotting, Western
Cell Culture Techniques
Cell Line, Tumor
Cyclohexanecarboxylic Acids / metabolism
Cyclohexanecarboxylic Acids / pharmacokinetics*
Female
Gabapentin
Gene Knockdown Techniques
Humans
Large Neutral Amino Acid-Transporter 1 / genetics
Large Neutral Amino Acid-Transporter 1 / metabolism*
Placenta / metabolism*
Pregnancy
Reverse Transcriptase Polymerase Chain Reaction
gamma-Aminobutyric Acid / metabolism
gamma-Aminobutyric Acid / pharmacokinetics*
|
| IF |
3.121
|
| リソース情報 |
| ヒト・動物細胞 |
BeWo |
