RRC ID 61777
Author Tsuda E, Goto M, Mochizuki S, Yano K, Kobayashi F, Morinaga T, Higashio K.
Title Isolation of a novel cytokine from human fibroblasts that specifically inhibits osteoclastogenesis.
Journal Biochem Biophys Res Commun
Abstract A factor which inhibits osteoclast-like cell formation was found in the conditioned medium of human embryonic lung fibroblasts, IMR-90. The factor, termed osteoclastogenesis inhibitory factor, OCIF, was purified to homogeneity. OCIF is a heparin-binding basic glycoprotein and has been isolated as a monomer with an apparent molecular weight (Mr) of 60,000 and a homodimer with a Mr of 120,000. The N-terminus of OCIF is blocked and the determination of internal amino acid sequences revealed that OCIF has no homology to known proteins. OCIF inhibited in a dose-dependent manner osteoclastogenesis elicited through three distinct signaling pathways stimulated by 1 alpha,25-dihydroxy vitamin D3, parathyroid hormone, and interleukin-11, respectively, in a dose range of 1 to 40 ng/ml (IC50 = 4 to 6 ng/ml). OCIF neither inhibits bone resorption by mature osteoclasts nor exerts any other biological activities. These data strongly suggest that OCIF is a novel cytokine which specifically inhibits osteoclastogenesis.
Volume 234(1)
Pages 137-42
Published 1997-5-8
DOI 10.1006/bbrc.1997.6603
PII S0006291X97966031
PMID 9168977
MeSH Acid Phosphatase / metabolism Animals Calcitriol / pharmacology Cell Line Chromatography, Affinity Chromatography, High Pressure Liquid Chromatography, Ion Exchange Culture Media, Conditioned Cytokines / chemistry Cytokines / isolation & purification* Cytokines / pharmacology* Electrophoresis, Polyacrylamide Gel Fibroblasts / chemistry* Humans Mice Molecular Weight Osteoclasts / cytology* Osteoclasts / drug effects Parathyroid Hormone / pharmacology Serine Endopeptidases / metabolism
IF 2.985
Human and Animal Cells ST2(RCB0224) MC3T3-G2/PA6(RCB1127)