論文 - 詳細
| RRC ID | 61784 |
|---|---|
| 著者 | Shimura Y, Suzuki J, Muraoka M, Kasuya MC, Matsuoka K, Hatanaka K. |
| タイトル | Large scale biosynthesis of ganglioside analogues by RERF-LC-AI cells cultured in HYPERFlask. |
| ジャーナル | Prep Biochem Biotechnol |
| Abstract |
The efficient production of ganglioside analogues was accomplished using RERF-LC-AI cells cultured in HYPERFlask (High Yield PERformance Flask). Eight kinds of ganglioside analogues (GM3, GM2, sialylparagloboside, GD3, di-sialylated lacto-N-tetraose, and another three kinds of analogues with intricate structures) were synthesized by the saccharide primer method using lung squamous-cell carcinoma line RERF-LC-AI and 12-azidododecyl β-lactoside primer. The yield for each analogue obtained using HYPERFlask was higher than yields obtained from 100-mm dishes. |
| 巻・号 | 42(4) |
| ページ | 378-92 |
| 公開日 | 2012-1-1 |
| DOI | 10.1080/10826068.2011.627971 |
| PMID | 22708814 |
| MeSH | Azides / metabolism Biotechnology / methods* Carbohydrate Sequence Cell Culture Techniques / methods* Cell Line, Tumor Gangliosides / chemistry* Gangliosides / isolation & purification Gangliosides / metabolism* Humans Lactose / analogs & derivatives Lactose / metabolism Lung / cytology* Molecular Sequence Data |
| IF | 1.415 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | RERF-LC-AI(RCB0444) |