RRC ID 61792
Author Uzawa K, Kasamatsu A, Saito T, Takahara T, Minakawa Y, Koike K, Yamatoji M, Nakashima D, Higo M, Sakamoto Y, Shiiba M, Tanzawa H.
Title Long-term culture of human odontoma-derived cells with a Rho kinase inhibitor.
Journal Exp Cell Res
Abstract Because of cellular senescence/apoptosis, no effective culture systems are available to maintain replication of cells from odontogenic tumors especially for odontoma, and, thus, the ability to isolate human odontoma-derived cells (hODCs) for functional studies is needed. The current study was undertaken to develop an approach to isolate hODCs and fully characterize the cells in vitro. The hODCs were cultured successfully with a Rho-associated protein kinase inhibitor (Y-27632) for an extended period with stabilized lengths of the telomeres to sustain a similar phenotype/property as the primary tumoral cells. While the hODCs showed stable long-term expansion with expression of major dental epithelial markers including dentin sialophosphoprotein (DSPP) even in the three-dimensional microenvironment, they lack the specific markers for the characteristics of stem cells. Moreover, cells from dental pulp showed significant up-regulation of DSPP when co-cultured with the hODCs, while control fibroblasts with the hODCs did not. Taken together, we propose that the hODCs can be isolated and expanded over the long term with Y-27632 to investigate not only the development of the hODCs but also other types of benign human tumors.
Volume 347(1)
Pages 232-240
Published 2016-9-10
DOI 10.1016/j.yexcr.2016.08.005
PII S0014-4827(16)30238-5
PMID 27514999
MeSH Amides Apoptosis / drug effects Calcification, Physiologic / drug effects Cell Differentiation / drug effects Cell Proliferation / drug effects Cellular Senescence / drug effects Coculture Techniques Extracellular Matrix Proteins / genetics Extracellular Matrix Proteins / metabolism Humans Odontogenesis / drug effects Odontogenesis / genetics Odontoma / enzymology* Odontoma / genetics Odontoma / pathology* Osteogenesis / drug effects Osteogenesis / genetics Phosphoproteins / genetics Phosphoproteins / metabolism Protein Kinase Inhibitors / pharmacology* Pyridines RNA, Messenger / genetics RNA, Messenger / metabolism Sialoglycoproteins / genetics Sialoglycoproteins / metabolism Telomerase / genetics Telomerase / metabolism Telomere Homeostasis / drug effects Time Factors Tumor Cells, Cultured rho-Associated Kinases / antagonists & inhibitors* rho-Associated Kinases / metabolism
IF 3.383
Human and Animal Cells human skin fibroblasts