| RRC ID |
61819
|
| Author |
Fukuda T, Ochi H, Sunamura S, Haiden A, Bando W, Inose H, Okawa A, Asou Y, Takeda S.
|
| Title |
MicroRNA-145 regulates osteoblastic differentiation by targeting the transcription factor Cbfb.
|
| Journal |
FEBS Lett
|
| Abstract |
Osteoblastic differentiation is regulated by various factors, including hormones and transcription factors. Runt-related transcription factor 2 (Runx2) is an essential player in osteoblastogenesis and transactivates its molecular target by creating a protein complex with its hetero-dimeric partner core binding factor beta (Cbfb). However, the molecular regulation of Cbfb expression remains unknown. Here, we identified miR-145 as a crucial regulator of Cbfb expression. The expression of miR-145 increased during osteoblastogenesis, indicating that miR-145 works as an inhibitor of osteoblastogenesis. Stable expression of miR-145 decreased endogenous Cbfb expression and inhibited osteoblastogenesis, in cooperation with miR-34c. Furthermore, miR-145 decreased bone regeneration in vivo. Our results indicate that miR-145 physiologically regulates osteoblast differentiation and bone formation via Cbfb expression by forming a regulatory microRNA network.
|
| Volume |
589(21)
|
| Pages |
3302-8
|
| Published |
2015-10-24
|
| DOI |
10.1016/j.febslet.2015.09.024
|
| PII |
S0014-5793(15)00873-X
|
| PMID |
26450370
|
| MeSH |
3T3 Cells
Animals
Bone Regeneration
Cell Differentiation
Cell Line
Core Binding Factor beta Subunit / genetics*
Core Binding Factor beta Subunit / metabolism
Gene Expression Regulation
HEK293 Cells
Humans
Mice
MicroRNAs / metabolism*
Osteoblasts / physiology*
Promoter Regions, Genetic
|
| IF |
3.057
|
| Resource |
| Human and Animal Cells |
UCB408E6E7TERT-33(RCB2080)
GE1(RCB1709) |
