RRC ID 6184
Author Sakamoto K, Tamamura Y, Katsube K, Yamaguchi A.
Title Zfp64 participates in Notch signaling and regulates differentiation in mesenchymal cells.
Journal J Cell Sci
Abstract Notch signaling is required for multiple aspects of tissue and cell differentiation. In this study, we identified zinc finger protein 64 (Zfp64) as a novel coactivator of Notch1. Zfp64 is associated with the intracellular domain of Notch1, recruited to the promoters of the Notch target genes Hes1 and Hey1, and transactivates them. Zfp64 expression is under the control of Runx2, and is upregulated by direct transactivation of its promoter. Zfp64 suppresses the myogenic differentiation of C2C12 cells and promotes their osteoblastic differentiation. Our data demonstrate two functions of Zfp64: (1) it is a downstream target of Runx2 and, (2) its cognate protein acts as a coactivator of Notch1, which suggests that Zfp64 mediates mesenchymal cell differentiation by modulating Notch signaling.
Volume 121(Pt 10)
Pages 1613-23
Published 2008-5-15
DOI 10.1242/jcs.023119
PII jcs.023119
PMID 18430783
MeSH Animals Base Sequence Basic Helix-Loop-Helix Transcription Factors / metabolism Cell Cycle Proteins / metabolism Cell Differentiation* Cell Line Core Binding Factor Alpha 1 Subunit / metabolism* DNA-Binding Proteins / metabolism* Homeodomain Proteins / metabolism Humans Mesenchymal Stem Cells / cytology* Mesenchymal Stem Cells / metabolism Mesoderm / metabolism* Mice Molecular Sequence Data Nuclear Proteins / metabolism* Receptor, Notch1 / metabolism* Signal Transduction Transcription Factor HES-1 Transcription Factors / metabolism* Transcriptional Activation Up-Regulation
IF 4.573
Times Cited 22
DNA material pCMX-N/RBP-J (RDB03021)