| RRC ID |
61897
|
| 著者 |
Mello A, Hong Z, Rossi AM, Luan L, Farina M, Querido W, Eon J, Terra J, Balasundaram G, Webster T, Feinerman A, Ellis DE, Ketterson JB, Ferreira CL.
|
| タイトル |
Osteoblast proliferation on hydroxyapatite thin coatings produced by right angle magnetron sputtering.
|
| ジャーナル |
Biomed Mater
|
| Abstract |
Right angle magnetron sputtering (RAMS) was used to produce hydroxyapatite (HA) film coatings on pure titanium substrates and oriented silicon wafer (Si(0 0 1)) substrates with flat surfaces as well as engineered surfaces having different forms. Analyses using synchrotron XRD, AFM, XPS, FTIR and SEM with EDS showed that as-sputtered thin coatings consist of highly crystalline hydroxyapatite. The HA coatings induced calcium phosphate precipitation when immersed in simulated body fluid, suggesting in vivo bioactive behavior. In vitro experiments, using murine osteoblasts, showed that cells rapidly adhere, spread and proliferate over the thin coating surface, while simultaneously generating strong in-plane stresses, as observed on SEM images. Human osteoblasts were seeded at a density of 2500 cells cm(-2) on silicon and titanium HA coated substrates by RAMS. Uncoated glass was used as a reference substrate for further counting of cells. The highest proliferation of human osteoblasts was achieved on HA RAMS-coated titanium substrates. These experiments demonstrate that RAMS is a promising coating technique for biomedical applications.
|
| 巻・号 |
2(2)
|
| ページ |
67-77
|
| 公開日 |
2007-6-1
|
| DOI |
10.1088/1748-6041/2/2/003
|
| PII |
S1748-6041(07)42952-7
|
| PMID |
18458438
|
| MeSH |
Animals
Body Fluids / chemistry*
Cell Proliferation
Cells, Cultured
Coated Materials, Biocompatible / chemistry*
Hot Temperature
Humans
Materials Testing
Mice
Osteoblasts / cytology*
Osteoblasts / physiology*
Osteogenesis / physiology*
Surface Properties
Titanium / chemistry*
|
| IF |
3.174
|
| リソース情報 |
| ヒト・動物細胞 |
MC3T3-E1(RCB1126) |
