RRC ID 61898
Author Hayashi C, Hasegawa U, Saita Y, Hemmi H, Hayata T, Nakashima K, Ezura Y, Amagasa T, Akiyoshi K, Noda M.
Title Osteoblastic bone formation is induced by using nanogel-crosslinking hydrogel as novel scaffold for bone growth factor.
Journal J Cell Physiol
Abstract Bone regeneration for the defects in revision surgery of joint replacement is an increasingly important issue. To repair bone defects, bone cell activation by growth factors using synthetic resorbable scaffold is a useful and safe option. We examine the efficiency of nanogel-crosslinking hydrogel as a novel synthetic scaffold for BMP to stimulate osteoblasts and to induce bone formation. Cholesterol-bearing pullulan nanogel-crosslinking hydrogel (CHPA/Hydrogel) was used to deliver BMP. The CHPA hydrogel pellets were implanted in vivo. Single implantation of CHPA/hydrogel containing low amounts of BMP induced osteoblastic activation and new bone formation in vivo. Furthermore, nanogel in a disc shape established recruitment of osteoblastic cells that vigorously formed bone to heal the calvarial defects, which did not heal spontaneously without it. In conclusion, CHPA/hydrogel serves as an efficient and versatile scaffold for the stimulation of osteoblasts to form bone and to repair defects via delivery of BMP.
Volume 220(1)
Pages 1-7
Published 2009-7-1
DOI 10.1002/jcp.21760
PMID 19301257
MeSH Animals Bone Morphogenetic Protein 2 / chemistry Bone Morphogenetic Protein 2 / pharmacology* Bone Regeneration / drug effects Cholesterol / chemistry Cross-Linking Reagents / chemistry* Disease Models, Animal Dose-Response Relationship, Drug Drug Carriers* Drug Implants Glucans / chemistry Humans Hydrogels* Kinetics Male Mice Mice, Inbred ICR Nanogels Ossification, Heterotopic / physiopathology Osteoblasts / drug effects* Osteoblasts / pathology Osteogenesis / drug effects* Polyethylene Glycols / chemistry* Polyethyleneimine / chemistry* Recombinant Proteins / pharmacology Skull / drug effects Skull / physiopathology Skull / surgery Solubility Tissue Engineering* Tissue Scaffolds* X-Ray Microtomography
IF 5.546
Human and Animal Cells MC3T3-E1(RCB1126)