| RRC ID |
61920
|
| Author |
Baba T, Sakamoto Y, Kasamatsu A, Minakawa Y, Yokota S, Higo M, Yokoe H, Ogawara K, Shiiba M, Tanzawa H, Uzawa K.
|
| Title |
Persephin: A potential key component in human oral cancer progression through the RET receptor tyrosine kinase-mitogen-activated protein kinase signaling pathway.
|
| Journal |
Mol Carcinog
|
| Abstract |
Persephin (PSPN) is a neurotrophic factor of the glial cell line-derived neurotrophic factor (GDNF) family that promotes survival of multiple populations of neurons. Little is known about the relevance of PSPN in human malignancy including oral squamous cell carcinoma (OSCC). This study was undertaken to evaluate PSPN mRNA and protein expression by analyzing cellular proliferation and the cell cycle in PSPN knockdown cells in vitro. PSPN mRNA and protein were significantly (P < 0.05) up-regulated in OSCC-derived cells compared with human normal oral keratinocytes (n = 7). Cellular proliferation decreased significantly (P < 0.05) in PSPN knockdown cells with reduced receptor tyrosine kinase (RTK) signaling, and cell-cycle arrest at the G1 phase resulted from up-regulation of the cyclin-dependent kinase inhibitors (p21(Cip1) , p27(Kip1) , p15(INK4B) , and p16(INK4A) ). Furthermore, the PSPN protein expression in 101 primary OSCCs was significantly (P < 0.05) higher than in normal counterparts. Among the clinical variables analyzed, overexpression of PSPN also was related closely (P < 0.05) to tumoral size. Our results suggested that PSPN is a possible key regulator of OSCC progression via PSPN-RET-mitogen-activated protein kinase activation and that PSPN overexpression may have diagnostic potential for OSCC.
|
| Volume |
54(8)
|
| Pages |
608-17
|
| Published |
2015-8-1
|
| DOI |
10.1002/mc.22127
|
| PMID |
24375483
|
| MeSH |
Adult
Aged
Aged, 80 and over
Carcinoma, Squamous Cell / genetics
Carcinoma, Squamous Cell / metabolism
Carcinoma, Squamous Cell / pathology*
Cell Cycle Checkpoints
Cell Line, Tumor
Disease Progression
Humans
MAP Kinase Signaling System*
Middle Aged
Mouth Neoplasms / genetics
Mouth Neoplasms / metabolism
Mouth Neoplasms / pathology*
Nerve Tissue Proteins / genetics*
Nerve Tissue Proteins / metabolism*
Receptor Protein-Tyrosine Kinases / metabolism
Up-Regulation
|
| IF |
3.825
|
| Resource |
| Human and Animal Cells |
Ca9-22(RCB1976)
Sa3(RCB0980)
HSC-2(RCB1945)
HSC-4(RCB1902) |
