論文 - 詳細
| RRC ID | 61947 |
|---|---|
| 著者 | Yamaguchi H, Kojima H, Takezawa T. |
| タイトル | Predictive performance of the Vitrigel-eye irritancy test method using 118 chemicals. |
| ジャーナル | J Appl Toxicol |
| Abstract |
We recently developed a novel Vitrigel-eye irritancy test (EIT) method. The Vitrigel-EIT method is composed of two parts, i.e., the construction of a human corneal epithelium (HCE) model in a collagen vitrigel membrane chamber and the prediction of eye irritancy by analyzing the time-dependent profile of transepithelial electrical resistance values for 3 min after exposing a chemical to the HCE model. In this study, we estimated the predictive performance of Vitrigel-EIT method by testing a total of 118 chemicals. The category determined by the Vitrigel-EIT method in comparison to the globally harmonized system classification revealed that the sensitivity, specificity and accuracy were 90.1%, 65.9% and 80.5%, respectively. Here, five of seven false-negative chemicals were acidic chemicals inducing the irregular rising of transepithelial electrical resistance values. In case of eliminating the test chemical solutions showing pH 5 or lower, the sensitivity, specificity and accuracy were improved to 96.8%, 67.4% and 84.4%, respectively. Meanwhile, nine of 16 false-positive chemicals were classified irritant by the US Environmental Protection Agency. In addition, the disappearance of ZO-1, a tight junction-associated protein and MUC1, a cell membrane-spanning mucin was immunohistologically confirmed in the HCE models after exposing not only eye irritant chemicals but also false-positive chemicals, suggesting that such false-positive chemicals have an eye irritant potential. These data demonstrated that the Vitrigel-EIT method could provide excellent predictive performance to judge the widespread eye irritancy, including very mild irritant chemicals. We hope that the Vitrigel-EIT method contributes to the development of safe commodity chemicals. Copyright © 2015 The Authors. Journal of Applied Toxicology published by John Wiley & Sons Ltd. |
| 巻・号 | 36(8) |
| ページ | 1025-37 |
| 公開日 | 2016-8-1 |
| DOI | 10.1002/jat.3254 |
| PMID | 26472347 |
| PMC | PMC5057344 |
| MeSH | Animal Testing Alternatives / methods* Cells, Cultured Collagen / chemistry Electric Impedance Endothelial Cells / drug effects Endothelial Cells / metabolism Epithelium, Corneal / cytology Epithelium, Corneal / drug effects Epithelium, Corneal / metabolism Eye / drug effects* Eye / metabolism Humans Hydrogen-Ion Concentration Irritants / toxicity* Models, Biological Mucin-1 / genetics Mucin-1 / metabolism Reproducibility of Results Sensitivity and Specificity Tight Junctions / drug effects Tight Junctions / metabolism Toxicity Tests / methods* United States United States Environmental Protection Agency |
| IF | 2.997 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | HCE-T(RCB2280) |