RRC ID 61959
著者 Xi J, Kong L, Gao Y, Gong Y, Zhao N, Zhang X.
タイトル Properties of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) films modified with polyvinylpyrrolidone and behavior of MC3T3-E1 osteoblasts cultured on the blended films.
ジャーナル J Biomater Sci Polym Ed
Abstract A series of composite films of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx) modified with polyvinylpyrrolidone (PVP) was prepared by varying the ratio of constituents, and their properties and cytocompatibility were evaluated. The hydrophilicity of the blended materials surfaces increased and the amounts of fibronectin and laminin adsorbed on the materials surface increased remarkably compared with PHBHHx. FT-IR spectra of the blended films showed a new band, implying that a surface physical interpenetrating network structure had formed. Scanning electron microscopy showed that there were dense pits and holes on the blended films surface. For the films of PHBHHx with 20 wt% and 40 wt% PVP, MTT assay indicated that PVP enhanced cell adhesion and proliferation, but that the effects were impaired by excessive PVP. The results suggested that proper addition of PVP increased the cytocompatibility of PHBHHx because the material surface had increased hydrophilicity and presented an appropriate morphology.
巻・号 16(11)
ページ 1395-408
公開日 2005-1-1
DOI 10.1163/156856205774472344
PMID 16370240
MeSH 3-Hydroxybutyric Acid / chemistry* 3T3 Cells Adsorption Animals Biocompatible Materials Caproates / chemistry* Cell Adhesion Cell Proliferation Cells, Cultured Coloring Agents / pharmacology Extracellular Matrix / metabolism Fibronectins / chemistry Hydroxybutyrates / chemistry Laminin / chemistry Materials Testing Mice Microscopy, Electron, Scanning Models, Chemical Molecular Conformation Osteoblasts / cytology* Polyesters / chemistry Polymers / chemistry Povidone / chemistry* Spectrometry, X-Ray Emission Spectroscopy, Fourier Transform Infrared Surface Properties Tetrazolium Salts / pharmacology Thiazoles / pharmacology Tissue Engineering
IF 2.69
リソース情報
ヒト・動物細胞 MC3T3-E1(RCB1126)