RRC ID |
61959
|
著者 |
Xi J, Kong L, Gao Y, Gong Y, Zhao N, Zhang X.
|
タイトル |
Properties of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) films modified with polyvinylpyrrolidone and behavior of MC3T3-E1 osteoblasts cultured on the blended films.
|
ジャーナル |
J Biomater Sci Polym Ed
|
Abstract |
A series of composite films of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx) modified with polyvinylpyrrolidone (PVP) was prepared by varying the ratio of constituents, and their properties and cytocompatibility were evaluated. The hydrophilicity of the blended materials surfaces increased and the amounts of fibronectin and laminin adsorbed on the materials surface increased remarkably compared with PHBHHx. FT-IR spectra of the blended films showed a new band, implying that a surface physical interpenetrating network structure had formed. Scanning electron microscopy showed that there were dense pits and holes on the blended films surface. For the films of PHBHHx with 20 wt% and 40 wt% PVP, MTT assay indicated that PVP enhanced cell adhesion and proliferation, but that the effects were impaired by excessive PVP. The results suggested that proper addition of PVP increased the cytocompatibility of PHBHHx because the material surface had increased hydrophilicity and presented an appropriate morphology.
|
巻・号 |
16(11)
|
ページ |
1395-408
|
公開日 |
2005-1-1
|
DOI |
10.1163/156856205774472344
|
PMID |
16370240
|
MeSH |
3-Hydroxybutyric Acid / chemistry*
3T3 Cells
Adsorption
Animals
Biocompatible Materials
Caproates / chemistry*
Cell Adhesion
Cell Proliferation
Cells, Cultured
Coloring Agents / pharmacology
Extracellular Matrix / metabolism
Fibronectins / chemistry
Hydroxybutyrates / chemistry
Laminin / chemistry
Materials Testing
Mice
Microscopy, Electron, Scanning
Models, Chemical
Molecular Conformation
Osteoblasts / cytology*
Polyesters / chemistry
Polymers / chemistry
Povidone / chemistry*
Spectrometry, X-Ray Emission
Spectroscopy, Fourier Transform Infrared
Surface Properties
Tetrazolium Salts / pharmacology
Thiazoles / pharmacology
Tissue Engineering
|
IF |
2.69
|
リソース情報 |
ヒト・動物細胞 |
MC3T3-E1(RCB1126) |