論文 - 詳細
| RRC ID | 61966 |
|---|---|
| 著者 | Kato T, Miyagawa A, Kasuya MC, Ito A, Hatanaka K. |
| タイトル | Purification by centrifugal partition chromatography of amphiphilic compounds, glycolipids and pseudo-glycolipids synthesized by using cells. |
| ジャーナル | J Chromatogr A |
| Abstract |
Centrifugal partition chromatography (CPC) was applied to separate amphiphilic glycolipids and pseudo-glycolipids synthesized by using cells. Neutral and acidic lipid fractions were isolated by CPC under suitable conditions respectively. Separation of neutral lipid, Gb3-type and Gb4-type oligosaccharide synthesized by using cells, was performed with a two-phase solvent system composed of chloroform-methanol-water at a volume ratio of 5:6:4. On the other hand, separation of acidic lipid, GM3-type oligosaccharide synthesized by using cells, and ganglioside extracted from rat brain were performed with a two-phase solvent system composed of butanol-ethanol-1% acetic acid at a volume ratio of 4:1:5. 8.3mg of Gb3 analogue, 5.1mg of Gb4 analogue, and 19.5mg of GM3 analogue were purified from 3.2l of culture medium obtained by incubation of African green-monkey kidney (Vero) cells with 50 microM n-dodecyl beta-lactoside using CPC. |
| 巻・号 | 1178(1-2) |
| ページ | 154-9 |
| 公開日 | 2008-1-18 |
| DOI | 10.1016/j.chroma.2007.11.094 |
| PII | S0021-9673(07)02078-X |
| PMID | 18082172 |
| MeSH | Animals Brain Cell Line, Tumor Chlorocebus aethiops Chromatography / methods G(M3) Ganglioside / analysis G(M3) Ganglioside / isolation & purification* Gangliosides / analysis Gangliosides / isolation & purification* Glycolipids / analysis Glycolipids / isolation & purification* Mice Oligosaccharides / analysis Oligosaccharides / isolation & purification* Rats Vero Cells |
| IF | 4.049 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | Vero(RCB0001) |