| RRC ID |
61992
|
| 著者 |
Mizuno H, Luo W, Tarusawa E, Saito YM, Sato T, Yoshimura Y, Itohara S, Iwasato T.
|
| タイトル |
NMDAR-regulated dynamics of layer 4 neuronal dendrites during thalamocortical reorganization in neonates.
|
| ジャーナル |
Neuron
|
| Abstract |
Thalamocortical (TC) connectivity is reorganized by thalamic inputs during postnatal development; however, the dynamic characteristics of TC reorganization and the underlying mechanisms remain unexplored. We addressed this question using dendritic refinement of layer 4 (L4) stellate neurons in mouse barrel cortex (barrel cells) as a model; dendritic refinement of L4 neurons is a critical component of TC reorganization through which postsynaptic L4 neurons acquire their dendritic orientation toward presynaptic TC axon termini. Simultaneous labeling of TC axons and individual barrel cell dendrites allowed in vivo time-lapse imaging of dendritic refinement in the neonatal cortex. The barrel cells reinforced the dendritic orientation toward TC axons by dynamically moving their branches. In N-methyl-D-aspartate receptor (NMDAR)-deficient barrel cells, this dendritic motility was enhanced, and the orientation bias was not reinforced. Our data suggest that L4 neurons have "fluctuating" dendrites during TC reorganization and that NMDARs cell autonomously regulate these dynamics to establish fine-tuned circuits.
|
| 巻・号 |
82(2)
|
| ページ |
365-79
|
| 公開日 |
2014-4-16
|
| DOI |
10.1016/j.neuron.2014.02.026
|
| PII |
S0896-6273(14)00157-3
|
| PMID |
24685175
|
| MeSH |
Animals
Animals, Newborn
Cerebral Cortex / cytology*
Cerebral Cortex / physiology
Dendrites / drug effects
Dendrites / physiology*
Excitatory Amino Acid Agonists / pharmacology
Excitatory Postsynaptic Potentials / drug effects
Excitatory Postsynaptic Potentials / genetics
Female
Gene Expression Regulation, Developmental / physiology
In Vitro Techniques
Male
Mice
Mice, Inbred C57BL
Mice, Transgenic
Models, Biological
N-Methylaspartate / pharmacology
Nerve Tissue Proteins / genetics
Nerve Tissue Proteins / metabolism*
Neural Pathways / growth & development
Neural Pathways / metabolism
Neurons / cytology*
Neurons / drug effects
Receptors, N-Methyl-D-Aspartate / genetics
Receptors, N-Methyl-D-Aspartate / metabolism*
Thalamus / physiology*
alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid / pharmacology
|
| IF |
14.415
|
| リソース情報 |
| 遺伝子材料 |
pK031.TRE-Cre (Supernova) (RDB15043) |
