RRC ID |
62008
|
Author |
Okada A, Tashiro K, Yamaguchi T, Kawabata K.
|
Title |
Selective Differentiation into Hematopoietic and Cardiac Cells from Pluripotent Stem Cells Based on the Expression of Cell Surface Markers.
|
Journal |
Methods Mol Biol
|
Abstract |
Flk1-expressing (+) mesodermal cells are useful source for the generation of hematopoietic cells and cardiomyocytes from pluripotent stem cells (PSCs). However, they have been reported as a heterogenous population that includes hematopoietic and cardiac progenitors. Therefore, to provide a method for a highly efficient production of hematopoietic cells and cardiomyocytes, cell surface markers are often used for separating these progenitors in Flk1(+) cells. Our recent study has shown that the expression of coxsackievirus and adenovirus receptor (CAR), a tight junction component molecule, could divide mouse and human PSC- and mouse embryo-derived Flk1(+) cells into Flk1(+)CAR(-) and Flk1(+)CAR(+) cells. Flk1(+)CAR(-) and Flk1(+)CAR(+) cells efficiently differentiated into hematopoietic cells and cardiomyocytes, respectively. These results indicate that CAR is a novel cell surface marker for separating PSC-derived Flk1(+) mesodermal cells into hematopoietic and cardiac progenitors. We herein describe a differentiation method from PSCs into hematopoietic cells and cardiomyocytes based on CAR expression.
|
Volume |
1341
|
Pages |
181-95
|
Published |
2016-1-1
|
DOI |
10.1007/7651_2015_232
|
PMID |
26138986
|
MeSH |
Animals
Cell Culture Techniques / methods*
Cell Differentiation*
Cell Line
Cells, Cultured
Hematopoietic Stem Cells / cytology*
Humans
Mesoderm / cytology
Mice
Myocytes, Cardiac / cytology*
Pluripotent Stem Cells / cytology*
Vascular Endothelial Growth Factor Receptor-2 / analysis
|
Resource |
Human and Animal Cells |
BRC6(AES0010)
OP9(RCB1124) |