Reference - Detail
| RRC ID | 62008 |
|---|---|
| Author | Okada A, Tashiro K, Yamaguchi T, Kawabata K. |
| Title | Selective Differentiation into Hematopoietic and Cardiac Cells from Pluripotent Stem Cells Based on the Expression of Cell Surface Markers. |
| Journal | Methods Mol Biol |
| Abstract |
Flk1-expressing (+) mesodermal cells are useful source for the generation of hematopoietic cells and cardiomyocytes from pluripotent stem cells (PSCs). However, they have been reported as a heterogenous population that includes hematopoietic and cardiac progenitors. Therefore, to provide a method for a highly efficient production of hematopoietic cells and cardiomyocytes, cell surface markers are often used for separating these progenitors in Flk1(+) cells. Our recent study has shown that the expression of coxsackievirus and adenovirus receptor (CAR), a tight junction component molecule, could divide mouse and human PSC- and mouse embryo-derived Flk1(+) cells into Flk1(+)CAR(-) and Flk1(+)CAR(+) cells. Flk1(+)CAR(-) and Flk1(+)CAR(+) cells efficiently differentiated into hematopoietic cells and cardiomyocytes, respectively. These results indicate that CAR is a novel cell surface marker for separating PSC-derived Flk1(+) mesodermal cells into hematopoietic and cardiac progenitors. We herein describe a differentiation method from PSCs into hematopoietic cells and cardiomyocytes based on CAR expression. |
| Volume | 1341 |
| Pages | 181-95 |
| Published | 2016-1-1 |
| DOI | 10.1007/7651_2015_232 |
| PMID | 26138986 |
| MeSH | Animals Cell Culture Techniques / methods* Cell Differentiation* Cell Line Cells, Cultured Hematopoietic Stem Cells / cytology* Humans Mesoderm / cytology Mice Myocytes, Cardiac / cytology* Pluripotent Stem Cells / cytology* Vascular Endothelial Growth Factor Receptor-2 / analysis |
| Altmetric score |
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 2 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | BRC6(AES0010) OP9(RCB1124) |