RRC ID 62036
Author Hasehira K, Hirabayashi J, Tateno H.
Title Structural and quantitative evidence of α2-6-sialylated N-glycans as markers of the differentiation potential of human mesenchymal stem cells.
Journal Glycoconj J
Abstract Human somatic stem cells such as mesenchymal stem cells (hMSCs) have the capacity to differentiate into mesenchymal tissue lineages and to alter immune regulatory functions. As such, they hold promise for use in stem cell-based therapies. However, no method is currently available to evaluate the actual differentiation capacity of hMSCs prior to cell transplantation. Previously, we performed a comprehensive glycan profiling of adipose-derived hMSCs using high-density lectin microarray and demonstrated that α2-6-sialylation is a marker of the differentiation potential of these cells. Nevertheless, no information was available about the structural details of these of α2-6-sialylated glycans. Here we used high performance liquid chromatography (HPLC) analysis combined with mass spectrometry (MS) to perform a structural and quantitative glycome analysis targeting both N- and O-glycans derived from early (with differentiation ability) and late (without differentiation ability) passages of adipose tissue-derived hMSCs. Findings in these cells were compared with those from human induced pluripotent stem cells (hiPSCs), human dermal fibroblasts (hFibs) and cartilage tissue-derived chondrocytes. A higher percentage of α2-6-sialylated N-glycans was detected in early passage cells (24-28 % of sialylated N-glycans) compared with late passage cells (13-15 %). A major α2-6-sialylated N-glycan structure detected in adipose-derived hMSCs was that of mono-sialylated biantennary N-glycan. Similar results were obtained for the cartilage tissue-derived chondrocytes, Yub621c (28 % for passage 7 and 5 % for passage 28). In contrast, no significant differences were observed between early and late passage hMSCs with respect to α2-6-sialylated O-glycan percentages. These results demonstrate that levels of α2-6-sialylated N-glycans, but not O-glycans, could be used as markers of the differential potential of hMSCs.
Volume 34(6)
Pages 797-806
Published 2017-12-1
DOI 10.1007/s10719-016-9699-6
PII 10.1007/s10719-016-9699-6
PMID 27314244
PMC PMC5711977
MeSH Adipose Tissue / cytology Cell Differentiation* Cells, Cultured Chondrocytes / cytology Chondrocytes / metabolism Fibroblasts / cytology Fibroblasts / metabolism Humans Induced Pluripotent Stem Cells / cytology Induced Pluripotent Stem Cells / metabolism Mesenchymal Stem Cells / cytology Mesenchymal Stem Cells / metabolism* Polysaccharides / chemistry Polysaccharides / metabolism* Sialic Acids / metabolism*
IF 2.197
Resource
Human and Animal Cells 201B7(HPS0063)