| RRC ID |
62040
|
| 著者 |
Yamada A, Kawano K, Harashima N, Niiya F, Nagai K, Kobayashi T, Mine T, Ushijima K, Nishida T, Itoh K.
|
| タイトル |
Study of HLA class I restriction and the directed antigens of cytotoxic T lymphocytes at the tumor sites of ovarian cancer.
|
| ジャーナル |
Cancer Immunol Immunother
|
| Abstract |
The molecular basis of T-cell-mediated recognition of ovarian cancer cells remains to be fully addressed. In this study we investigated HLA class I restriction and directed antigens of cytotoxic T lymphocytes (CTL) at the sites of ovarian cancer. Three HLA-class-I-restricted CTL lines were established from the tumor sites of ovarian cancer by culturing tumor-infiltrating lymphocytes or tumor-associated ascitic lymphocytes with interleukin-2: (1) HLA-A2402-restricted and ovarian-adenocarcinoma-specific CTL, (2) HLA-A2-restricted CTL recognizing histologically different cancers, and (3) HLA-B52-restricted and ovarian-cancer-specific CTL. HLA-A0201, HLA-A0206 and HLA-A0207 tumor cells were lysed by the HLA-A2-restricted CTL. HLA-B52 restriction of the third CTL line was confirmed by the transfection of HLA-B5201 cDNA into the tumor cells. The HLA-A2-restricted CTL recognized the SART-1, but not the MAGE-1 or MAGE-3 antigen. These results may facilitate a better understanding of the molecular basis of tumor-specific immunity at the tumor site of ovarian cancer.
|
| 巻・号 |
48(2-3)
|
| ページ |
147-52
|
| 公開日 |
1999-1-1
|
| DOI |
10.1007/s002620050559
|
| PMID |
10414469
|
| MeSH |
Animals
Antigens, Neoplasm / immunology*
COS Cells
Female
Histocompatibility Antigens Class I / physiology*
Humans
Interferon-gamma / biosynthesis
Melanoma-Specific Antigens
Neoplasm Proteins / immunology
Ovarian Neoplasms / immunology*
Ribonucleoproteins, Small Nuclear*
T-Lymphocytes, Cytotoxic / immunology*
Tumor Cells, Cultured
|
| IF |
5.442
|
| リソース情報 |
| ヒト・動物細胞 |
OMC-3(RCB0755)
HTOA(RCB0692) |
