論文 - 詳細
| RRC ID | 62060 |
|---|---|
| 著者 | Kwon HJ. |
| タイトル | TGF-β but not BMP signaling induces prechondrogenic condensation through ATP oscillations during chondrogenesis. |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
Although both TGF-β and BMP signaling enhance expression of adhesion molecules during chondrogenesis, TGF-β but not BMP signaling can initiate condensation of uncondensed mesenchymal cells. However, it remains unclear what causes the differential effects between TGF-β and BMP signaling on prechondrogenic condensation. Our previous report demonstrated that ATP oscillations play a critical role in prechondrogenic condensation. Thus, the current study examined whether ATP oscillations are associated with the differential actions of TGF-β and BMP signaling on prechondrogenic condensation. The result revealed that while both TGF-β1 and BMP2 stimulated chondrogenic differentiation, TGF-β1 but not BMP2 induced prechondrogenic condensation. It was also found that TGF-β1 but not BMP2 induced ATP oscillations and inhibition of TGF-β but not BMP signaling prevented insulin-induced ATP oscillations. Moreover, blockage of ATP oscillations inhibited TGF-β1-induced prechondrogenic condensation. In addition, TGF-β1-driven ATP oscillations and prechondrogenic condensation depended on Ca(2+) influx via voltage-dependent calcium channels. This study suggests that Ca(2+)-driven ATP oscillations mediate TGF-β-induced the initiation step of prechondrogenic condensation and determine the differential effects between TGF-β and BMP signaling on chondrogenesis. |
| 巻・号 | 424(4) |
| ページ | 793-800 |
| 公開日 | 2012-8-10 |
| DOI | 10.1016/j.bbrc.2012.07.041 |
| PII | S0006-291X(12)01324-1 |
| PMID | 22814106 |
| MeSH | Adenosine Triphosphate / metabolism* Animals Bone Morphogenetic Protein 2 / metabolism Bone Morphogenetic Protein 2 / pharmacology Bone Morphogenetic Proteins / metabolism* Bone Morphogenetic Proteins / pharmacology Calcium / metabolism* Cell Line, Tumor Chondrogenesis / drug effects Chondrogenesis / physiology* Collagen Type II / metabolism Mice Signal Transduction Transforming Growth Factor beta / metabolism* Transforming Growth Factor beta / pharmacology |
| IF | 2.985 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | ATDC5(RCB0565) |