| RRC ID |
62071
|
| Author |
Izumi M, Yanagi K, Mizuno T, Yokoi M, Kawasaki Y, Moon KY, Hurwitz J, Yatagai F, Hanaoka F.
|
| Title |
The human homolog of Saccharomyces cerevisiae Mcm10 interacts with replication factors and dissociates from nuclease-resistant nuclear structures in G(2) phase.
|
| Journal |
Nucleic Acids Res
|
| Abstract |
Mcm10 (Dna43), first identified in Saccharomyces cerevisiae, is an essential protein which functions in the initiation of DNA synthesis. Mcm10 is a nuclear protein that is localized to replication origins and mediates the interaction of the Mcm2-7 complex with replication origins. We identified and cloned a human cDNA whose product was structurally homologous to the yeast Mcm10 protein. Human Mcm10 (HsMcm10) is a 98-kDa protein of 874 amino acids which shows 23 and 21% overall similarity to Schizosaccharomyces pombe Cdc23 and S. cerevisiae Mcm10, respectively. The messenger RNA level of HsMcm10 increased at the G(1)/S-boundary when quiescent human NB1-RGB cells were induced to proliferate as is the case of many replication factors. HsMcm10 associated with nuclease-resistant nuclear structures throughout S phase and dissociated from it in G(2) phase. HsMcm10 associated with human Orc2 protein when overexpressed in COS-1 cells. HsMcm10 also interacted with Orc2, Mcm2 and Mcm6 proteins in the yeast two-hybrid system. These results suggest that HsMcm10 may function in DNA replication through the interaction with Orc and Mcm2-7 complexes.
|
| Volume |
28(23)
|
| Pages |
4769-77
|
| Published |
2000-12-1
|
| DOI |
10.1093/nar/28.23.4769
|
| PMID |
11095689
|
| PMC |
PMC115166
|
| MeSH |
Amino Acid Sequence
Animals
COS Cells
Cell Cycle / genetics
Cell Cycle Proteins / genetics*
Cell Cycle Proteins / metabolism
Cell Line
Cell Nucleus / metabolism*
Chromosomal Proteins, Non-Histone
DNA Replication
DNA, Complementary / chemistry
DNA, Complementary / genetics
DNA, Recombinant
DNA-Binding Proteins / genetics
DNA-Binding Proteins / metabolism*
Deoxyribonucleases / metabolism*
G2 Phase*
Gene Expression
HeLa Cells
Humans
Minichromosome Maintenance Proteins
Molecular Sequence Data
Origin Recognition Complex
Plasmids / genetics
Precipitin Tests
Protein Binding
RNA, Messenger / genetics
RNA, Messenger / metabolism
Saccharomyces cerevisiae / genetics
Saccharomyces cerevisiae Proteins*
Schizosaccharomyces pombe Proteins*
Sequence Alignment
Sequence Analysis, DNA
Sequence Homology, Amino Acid
Two-Hybrid System Techniques
|
| IF |
11.502
|
| Resource |
| Human and Animal Cells |
NB1RGB(RCB0222) |
| DNA material |
Human MCM10 cDNA (RDB06320) |
